Stojković Dejan, Drakulić Danijela, Gašić Uroš, Zengin Gokhan, Stevanović Milena, Rajčević Nemanja, Soković Marina
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković"- National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia.
Food Funct. 2020 Aug 1;11(8):7138-7151. doi: 10.1039/d0fo01595d. Epub 2020 Aug 4.
This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.
本研究探索了棘豆地上部分的化学特征，并进一步研究了其生物活性。提取物的化学分析显示存在63种不同的化合物：酚酸、黄酮糖苷和苷元、异黄酮糖苷和苷元以及其他相关化合物。我们的结果表明，该提取物对8株浮游细菌具有活性。它对金黄色葡萄球菌显示出抗生物膜潜力，并且能够在亚最小抑菌浓度下抑制金黄色葡萄球菌中金黄色色素的产生。通过几种测定法（磷钼酸法、DPPH法、ABTS法、CUPRAC法、FRAP法和金属螯合测定法）评估其抗氧化活性，结果表明该提取物呈现出剂量依赖性活性。该提取物实现了对乙酰胆碱酯酶、丁酰胆碱酯酶、淀粉酶、葡萄糖苷酶和酪氨酸酶的抑制，证明了其抗酶活性。通过结晶紫测定法确定了该提取物对人癌细胞系（HepG2、MCF-7、SiHa和A172）的抗增殖潜力。增殖标志物Ki67在A172胶质母细胞瘤细胞系中表达下调。
