骨桥蛋白诱导的长链非编码 RNA HOTAIR 的表达通过调节细胞增殖参与骨关节炎。
Osteopontin-induced lncRNA HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
机构信息
Department of Orthopedics, Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, No. 116, Huanghe Road, Shayibake District, 830000, Urumqi City, Xinjiang Province, People's Republic of China.
Department of Rehabilitation, the Ninth People's Hospital of Wuxi, 214000, Wuxi City, Jiangsu Province, People's Republic of China.
出版信息
BMC Geriatr. 2021 Jan 14;21(1):57. doi: 10.1186/s12877-020-01993-y.
BACKGROUND
Osteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. This study was carried out to investigate the role of HOTAIR in OA.
METHODS
Synovial fluid was extracted from both OA patients (n = 58) and healthy controls (n = 58). Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 µg/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay.
RESULTS
HOTAIR was upregulated in OA. A positive correlation between HOTAIR and osteopontin was observed. In the primary chondrocytes, osteopontin treatment increased HOTAIR expression, while HOTAIR overexpression and knockdown failed to significantly affect osteopontin expression. In addition, osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIRE knockdown decreased chondrocyte proliferation. In addition, HOTAIR knockdown reduced the effects of osteopontin treatment on cell proliferation.
CONCLUSIONS
Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
背景
骨桥蛋白在骨关节炎（OA）中通过调节破骨细胞的功能发挥关键作用。已知骨桥蛋白可以诱导长链非编码 RNA HOX 转录反义 RNA（HOTAIR）的表达，表明 HOTAIR 参与 OA。本研究旨在探讨 HOTAIR 在 OA 中的作用。
方法
从 OA 患者（n=58）和健康对照者（n=58）中提取滑液。通过 RT-qPCR 测定滑液中骨桥蛋白和 HOTAIR 的表达。用不同浓度（0、1、5 和 10μg/ml）的骨桥蛋白处理软骨细胞，然后通过 RT-qPCR 测定 HOTAIR 的表达。通过 CCK-8 测定分析骨桥蛋白和 HOTAIR 过表达以及 HOTAIR 敲低在调节软骨细胞增殖中的作用。
结果
OA 中 HOTAIR 上调。HOTAIR 与骨桥蛋白呈正相关。在原代软骨细胞中，骨桥蛋白处理增加 HOTAIR 表达，而过表达 HOTAIR 和敲低 HOTAIR 均不能显著影响骨桥蛋白表达。此外，骨桥蛋白和 HOTAIR 过表达增加软骨细胞增殖，而 HOTAIR 敲低降低软骨细胞增殖。此外，HOTAIR 敲低降低了骨桥蛋白处理对细胞增殖的影响。
结论
骨桥蛋白诱导的 HOTAIR 表达通过调节细胞增殖参与骨关节炎。
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