长链非编码 RNA HOTAIR/miR-130a-3p 轴在调节阿尔茨海默病认知和炎症中的自发运动作用。

The Involvement of lncRNA HOTAIR/miR-130a-3p Axis in the Regulation of Voluntary Exercise on Cognition and Inflammation of Alzheimer's Disease.

机构信息

School of Rehabilitation, Jiangsu Vocational College of Medicine, Yancheng, China.

Department of Rehabilitation Medicine, The Affiliated Sir Run Run Hospital of Nanjing Medical University, Nanjing, China.

出版信息

Am J Alzheimers Dis Other Demen. 2022 Jan-Dec;37:15333175221091424. doi: 10.1177/15333175221091424.

DOI:10.1177/15333175221091424

PMID:35442818

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10581116/

Abstract

BACKGROUND

Alzheimer's disease (AD) is an age-related neurodegenerative disease and exercises might mitigate the progression of AD. This investigation aimed to manifest the potential mechanism of exercises in AD.

METHODS

Morris water maze (MWM) test was conducted to evaluate the cognitive function in APP/PS1 mice. Quantitative real-time PCR was performed to detect the expression of HOTAIR and miR-130a-3p. The enzyme-linked immunosorbent assay was applied to appraise the concentration of IL-1β, IL-6, and TNF-α. A luciferase report experiment was implemented to substantiate the relationship between miR-130a-3p and HOTAIR.

RESULTS

Exercises contributed to the elevated expression of HOTAIR. The findings of MWM implied HOTAIR inhibited the impacts of voluntary exercises on escape latency, distance moved, percentage of time spent in the target quadrant, platform crossing times, and inflammation. MiR-130a-3p mediated the function of HOTAIR on cognitive ability and inflammation.

CONCLUSION

HOTAIR participated in the regulation of exercises on AD by sponging miR-130a-3p.

摘要

背景

阿尔茨海默病（AD）是一种与年龄相关的神经退行性疾病，运动可能减缓 AD 的进展。本研究旨在揭示运动在 AD 中的潜在机制。

方法

通过 Morris 水迷宫（MWM）试验评估 APP/PS1 小鼠的认知功能。采用实时定量 PCR 检测 HOTAIR 和 miR-130a-3p 的表达。酶联免疫吸附试验评估 IL-1β、IL-6 和 TNF-α的浓度。通过荧光素酶报告实验证实 miR-130a-3p 与 HOTAIR 之间的关系。