Oğuz Ahmet Regaib, Kaval Oğuz Elif, Özok Necati
Department of Science Faculty, Department of Biology, Van Yüzüncü Yıl University, Van, Turkey.
Department of Science Education, Van Yüzüncü Yıl University, Van, Turkey.
Toxicol Res (Camb). 2020 Nov 6;9(6):741-745. doi: 10.1093/toxres/tfaa076. eCollection 2020 Dec.
Lake Van fish ( Guldenstadt 1814) is the only fish that can adapt to the extreme conditions (pH 9.8 salinity 0.2% and alkalinity 151.2 meq/L) of Lake Van. In this study, it was aimed to determine the cytotoxic and genotoxic effects of chlorpyrifos (CPF) on Lake Van fish primary gill cell culture. Gill epithelium from Lake Van fish was isolated enzymatically and grown in primary culture on Leibovitz's L-15 medium. After different doses (0.01, 0.1, 1, and 10 μM) of CPF were applied to the gill cells, the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), and DNA damage levels (8-hydroxyguanine (8-OHdG)) were examined at the end of 24 and 48 h. It was determined that the TOS, MDA, and 8-OHdG levels increased in the cells exposed to high doses (1 and 10 μM) of CPF and the TAS was decreased ( < 0.05). It was revealed from this study that CPF administered at a dose higher than 1 μM can cause oxidative stress and DNA damage in the primary gill cell culture of Lake Van fish. In addition, the findings showed that Lake Van fish primary gill cell culture was useful in determining the effects of toxic substances likely to be the contaminants of a lake.
凡湖鱼（Guldenstadt，1814年）是唯一能适应凡湖极端条件（pH值9.8、盐度0.2%、碱度151.2毫当量/升）的鱼类。在本研究中，旨在确定毒死蜱（CPF）对凡湖鱼原代鳃细胞培养物的细胞毒性和遗传毒性作用。通过酶解法分离凡湖鱼的鳃上皮，并在Leibovitz's L - 15培养基上进行原代培养。在向鳃细胞施加不同剂量（0.01、0.1、1和10 μM）的CPF后，在24小时和48小时结束时检测总氧化剂状态（TOS）、总抗氧化剂状态（TAS）、丙二醛（MDA）和DNA损伤水平（8 - 羟基鸟嘌呤（8 - OHdG））。结果发现，暴露于高剂量（1和10 μM）CPF的细胞中，TOS、MDA和8 - OHdG水平升高，而TAS降低（P < 0.05）。该研究表明，高于1 μM剂量的CPF可在凡湖鱼原代鳃细胞培养物中引起氧化应激和DNA损伤。此外，研究结果表明，凡湖鱼原代鳃细胞培养物在确定可能作为湖泊污染物的有毒物质的影响方面是有用的。
