Allkja Jontana, Bjarnsholt Thomas, Coenye Tom, Cos Paul, Fallarero Adyary, Harrison Joe J, Lopes Susana P, Oliver Antonio, Pereira Maria Olivia, Ramage Gordon, Shirtliff Mark E, Stoodley Paul, Webb Jeremy S, Zaat Sebastian A J, Goeres Darla M, Azevedo Nuno Filipe
LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal.
Montana State University, Center for Biofilm Engineering, 366 Barnard Hall, Bozeman, MT, 59717, USA.
Biofilm. 2019 Nov 19;2:100010. doi: 10.1016/j.bioflm.2019.100010. eCollection 2020 Dec.
The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.
已发表研究缺乏可重复性是科学界面临的主要问题之一，生物膜微生物学领域也不例外。应对这一复杂问题的一个有效策略是使用最低信息指南。该策略为作者和审稿人提供了一份指南，说明稿件应包含哪些必要信息，以便对研究中的实验进行清晰解读并独立重复。经过生物膜领域国际团队之间的多次讨论，我们提出了一份关于微孔板中生物膜形成的分光光度法和荧光法评估的指南。本指南分为5个主要部分，每个部分都提出了一套全面的建议。最低信息指南的目的是提高科学交流的质量，从而增强生物膜微孔板检测中的实验室间可重复性。
