Thérien Maude, Kiesewalter Heiko T, Auria Emile, Charron-Lamoureux Vincent, Wibowo Mario, Maróti Gergely, Kovács Ákos T, Beauregard Pascale B
Centre SÈVE, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Canada.
Bacterial Interactions and Evolution Group, DTU Bioengineering, Technical University of Denmark, Kgs Lyngby, Denmark.
Biofilm. 2020 Mar 20;2:100021. doi: 10.1016/j.bioflm.2020.100021. eCollection 2020 Dec.
Secondary metabolites have an important impact on the biocontrol potential of soil-derived microbes. In addition, various microbe-produced chemicals have been suggested to impact the development and phenotypic differentiation of bacteria, including biofilms. The non-ribosomal synthesized lipopeptide of , surfactin, has been described to impact the plant promoting capacity of the bacterium. Here, we investigated the impact of surfactin production on biofilm formation of using the laboratory model systems; pellicle formation at the air-medium interface and architecturally complex colony development, in addition to plant root-associated biofilms. We found that the production of surfactin by is not essential for pellicle biofilm formation neither in the well-studied strain, NCIB 3610, nor in the newly isolated environmental strains, but lack of surfactin reduces colony expansion. Further, plant root colonization was comparable both in the presence or absence of surfactin synthesis. Our results suggest that surfactin-related biocontrol and plant promotion in strains are independent of biofilm formation.
次生代谢产物对土壤微生物的生物防治潜力具有重要影响。此外，各种微生物产生的化学物质已被证明会影响细菌的发育和表型分化，包括生物膜。已描述的由非核糖体合成的脂肽表面活性素会影响该细菌的植物促生能力。在此，我们使用实验室模型系统研究了表面活性素的产生对[细菌名称未给出]生物膜形成的影响；除了植物根相关生物膜外，还研究了在气-液界面形成菌膜以及结构复杂的菌落发育情况。我们发现，无论是在经过充分研究的菌株NCIB 3610中，还是在新分离的环境菌株中，表面活性素的产生对于菌膜生物膜的形成都不是必需的，但缺乏表面活性素会减少菌落扩展。此外，在有或没有表面活性素合成的情况下，植物根部定殖情况相当。我们的结果表明，[细菌名称未给出]菌株中与表面活性素相关的生物防治和植物促生作用与生物膜形成无关。
