Dentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dent Med Probl. 2020 Oct-Dec;57(4):433-440. doi: 10.17219/dmp/122004.
The myosin 1H (MYO1H) gene, located on chromosome 12, encodes the unconventional MYO1H protein, which is involved in the intracellular movement and morphology of chondrocytes, and plays a vital role in the prognathism or retrognathism of the mandible.
The objective of this study was to assess the relationship between the polymorphisms of the MYO1H gene and mandibular prognathism in the Iranian population.
The current project evaluated 64 patients with mandibular prognathism requiring orthognathic surgery and 60 controls with skeletal class I occlusion. Genome amplification was performed using specific primer pairs to assess the rs10850110 and rs11611277 polymorphisms of the MYO1H gene through the polymerase chain reaction (PCR). The restriction fragment length polymorphism (RFLP) technique was used to detect single-nucleotide polymorphisms. The data was analyzed using the χ2 test.
The patient and control groups were not significantly different in terms of age or gender (p > 0.05). In all, 3.1% of patients and 6.7% of controls had the rs10850110 polymorphism (p = 0.680), and 1.6% of patients and 5% of controls had the rs11611277 polymorphism (p = 0.602).
No significant correlation was noted between the rs10850110 and rs11611277 polymorphisms of the MYO1H gene and mandibular prognathism in the Iranian population. However, the lower frequency of these polymorphisms in the patient group suggests a possible association with mandibular retrognathism, which needs to be investigated with a larger sample size.
肌球蛋白 1H（MYO1H）基因位于 12 号染色体上，编码非典型肌球蛋白 1H 蛋白，该蛋白参与软骨细胞的细胞内运动和形态发生，对下颌前突或后缩的发生起着至关重要的作用。
本研究旨在评估 MYO1H 基因多态性与伊朗人群下颌前突的关系。
本研究纳入了 64 例需要正颌手术的下颌前突患者和 60 例骨性 I 类正常的对照者。使用特异性引物对基因组进行扩增，通过聚合酶链反应（PCR）评估 MYO1H 基因的 rs10850110 和 rs11611277 多态性。采用限制性片段长度多态性（RFLP）技术检测单核苷酸多态性。采用卡方检验进行数据分析。
患者组和对照组在年龄和性别方面无显著差异（p＞0.05）。rs10850110 多态性在患者中的发生率为 3.1%，在对照组中的发生率为 6.7%（p=0.680）；rs11611277 多态性在患者中的发生率为 1.6%，在对照组中的发生率为 5%（p=0.602）。
在伊朗人群中，MYO1H 基因的 rs10850110 和 rs11611277 多态性与下颌前突无显著相关性。然而，患者组中这些多态性的频率较低提示其可能与下颌后缩有关，这需要进一步扩大样本量进行研究。
