Sexton W L, Korthuis R J, Laughlin M H
Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri-Columbia 65211.
Am J Physiol. 1988 Feb;254(2 Pt 2):H274-8. doi: 10.1152/ajpheart.1988.254.2.H274.
The purpose of this study was to determine whether high-intensity exercise training increases the vascular flow capacity and capillary exchange capacity in isolated rat hindquarters. One group of 20 male Sprague-Dawley rats underwent six bouts of alternating running (2.5 min) and recovery (4.5 min), 5 days/wk at 60 m/min on a 15% grade for 6-10 wk (high-intensity exercise training), while a second group of 20 rats was cage confined (sedentary controls). Experiments were conducted in isolated, maximally dilated (papaverine) hindquarters perfused with an artificial plasma consisting of a Tyrode's solution containing 5 g/100 ml albumin. Vascular flow capacity was evaluated by measuring perfusate flow rate at four different perfusion pressures. Capillary exchange capacity was evaluated by measuring the capillary filtration coefficient. The efficacy of training was demonstrated by significant increases in succinate dehydrogenase activity in the white vastus lateralis and vastus intermedius muscles. Total hindquarter flow capacity was elevated 50-100% in the trained rats. This increased flow capacity was associated with an increase in the capillary filtration coefficient in the maximally vasodilated hindquarters, thus suggesting that the capillary exchange capacity was increased with high-speed exercise training. These results suggest that the vascular transport capacity in rat hindquarter muscles is significantly increased by high-intensity exercise training.
本研究的目的是确定高强度运动训练是否能增加离体大鼠后肢的血管血流量和毛细血管交换能力。一组20只雄性Sprague-Dawley大鼠每周5天，以60米/分钟的速度在15%坡度上进行6次交替跑步（2.5分钟）和恢复（4.5分钟），持续6 - 10周（高强度运动训练），而另一组20只大鼠则关在笼中（久坐对照组）。实验在灌注含5 g/100 ml白蛋白的Tyrode溶液的人工血浆、最大程度扩张（用罂粟碱）的离体后肢中进行。通过测量四个不同灌注压力下的灌注液流速来评估血管血流量。通过测量毛细血管滤过系数来评估毛细血管交换能力。训练的效果通过白外侧股肌和股中间肌中琥珀酸脱氢酶活性的显著增加得以证明。训练大鼠的后肢总血流量增加了50 - 100%。这种增加的血流量与最大程度血管扩张的后肢中毛细血管滤过系数的增加相关，因此表明高速运动训练增加了毛细血管交换能力。这些结果表明，高强度运动训练可显著增加大鼠后肢肌肉的血管运输能力。
