Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
J Zhejiang Univ Sci B. 2021 Jan 15;22(1):1-20. doi: 10.1631/jzus.B2000356.
Proteins are major functional units that are tightly connected to form complex and dynamic networks. These networks enable cells and organisms to operate properly and respond efficiently to environmental cues. Over the past decades, many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected. At the same time, rapid development in proteomics and mass spectrometry (MS) techniques makes it possible to identify interacting proteins and build comprehensive protein‒protein interaction networks. The resulting interactomes and networks have proven informative in the investigation of biological functions, such as in the field of DNA damage repair. In recent years, a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein‒protein interaction studies. As the technologies for enriching associated proteins and MS become more sophisticated, the studies of protein‒protein interactions are entering a new era. In this review, we summarize the strategies and recent developments for exploring protein‒protein interaction. In addition, we discuss the application of these tools in the investigation of protein‒protein interaction networks involved in DNA damage response and DNA repair.
蛋白质是紧密连接形成复杂和动态网络的主要功能单位。这些网络使细胞和生物体能够正常运作，并有效地对环境线索做出反应。在过去的几十年中，已经开发出许多生化方法来寻找蛋白质结合伴侣，以了解蛋白质网络是如何构建和连接的。同时，蛋白质组学和质谱（MS）技术的快速发展使得识别相互作用的蛋白质和构建全面的蛋白质相互作用网络成为可能。由此产生的相互作用组和网络在研究生物功能方面证明是有信息的，例如在 DNA 损伤修复领域。近年来，随着基于 MS 的蛋白质相互作用研究，已经发现了许多参与 DNA 损伤反应和 DNA 修复途径的蛋白质。随着富集相关蛋白质和 MS 的技术变得更加复杂，蛋白质相互作用的研究正在进入一个新时代。在这篇综述中，我们总结了探索蛋白质相互作用的策略和最新进展。此外，我们还讨论了这些工具在研究参与 DNA 损伤反应和 DNA 修复的蛋白质相互作用网络中的应用。
