Ding Zhaozhao, Zhou Mingliang, Zhou Zhengyu, Zhang Wenjie, Jiang Xinquan, Lu Xiaohong, Zuo Baoqi, Lu Qiang, Kaplan David L
Department of Prosthodontics, Oral Bioengineering and Regenerative Medicine Lab, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital Affiliated to Shanghai JiaoTong University, School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States.
ACS Biomater Sci Eng. 2019 Aug 12;5(8):4077-4088. doi: 10.1021/acsbiomaterials.9b00621. Epub 2019 Jul 10.
Strategies to control neovascularization in damaged tissues remain a key issue in regenerative medicine. Unlike most reported desferrioxamine (DFO)-loaded systems where DFO demonstrates a burst release, here we attain zero-order release behavior above 40 days. This outcome was achieved by blending DFO with silk nanofibers with special hydrophilic-hydrophobic properties. The special silk nanofibers showed strong physical binding capacity with DFO, avoiding chemical cross-linking. Using these new biomaterials in vivo in a rat wound model suggested that the DFO-loaded silk nanofiber hydrogel systems stimulated angiogenesis by the sustained release of DFO, but also facilitated cell migration and tissue ingrowth. These features resulted in faster formation of a blood vessel network in the wounds, as well improved healing when compared to the free DFO system. The DFO-loaded systems are also suitable for the regeneration of other tissues, such as nerve and bone, suggesting universality in the biomedical field.
控制受损组织中的新生血管形成的策略仍然是再生医学中的一个关键问题。与大多数报道的载有去铁胺（DFO）的系统不同，在那些系统中DFO表现出突释现象，而在这里我们实现了40天以上的零级释放行为。这一结果是通过将DFO与具有特殊亲水-疏水性质的丝纳米纤维混合而实现的。这种特殊的丝纳米纤维与DFO表现出很强的物理结合能力，避免了化学交联。在大鼠伤口模型中体内使用这些新型生物材料表明，载有DFO的丝纳米纤维水凝胶系统通过DFO的持续释放刺激血管生成，而且还促进细胞迁移和组织向内生长。这些特性导致伤口中血管网络形成更快，与游离DFO系统相比，愈合情况也得到改善。载有DFO的系统也适用于其他组织的再生，如神经和骨骼，这表明其在生物医学领域具有通用性。
