Chen Qing, Yan Miao, Hu Annan, Liang Bing, Lu Hongwei, Zhou Lei, Ma Yiqun, Jia Chao, Su Dihan, Kong Biao, Hong Wei, Jiang Libo, Dong Jian
Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, People's Republic of China.
Department of Chemistry, Fudan University, Shanghai, 200438, People's Republic of China.
Nanomicro Lett. 2024 Aug 1;16(1):259. doi: 10.1007/s40820-024-01469-3.
Surgery remains the standard treatment for spinal metastasis. However, uncontrolled intraoperative bleeding poses a significant challenge for adequate surgical resection and compromises surgical outcomes. In this study, we develop a thrombin (Thr)-loaded nanorobot-hydrogel hybrid superstructure by incorporating nanorobots into regenerated silk fibroin nanofibril hydrogels. This superstructure with superior thixotropic properties is injected percutaneously and dispersed into the spinal metastasis of hepatocellular carcinoma (HCC) with easy bleeding characteristics, before spinal surgery in a mouse model. Under near-infrared irradiation, the self-motile nanorobots penetrate into the deep spinal tumor, releasing Thr in a controlled manner. Thr-induced thrombosis effectively blocks the tumor vasculature and reduces bleeding, inhibiting tumor growth and postoperative recurrence with Au nanorod-mediated photothermal therapy. Our minimally invasive treatment platform provides a novel preoperative therapeutic strategy for HCC spinal metastasis effectively controlling intraoperative bleeding and tumor growth, with potentially reduced surgical complications and enhanced operative outcomes.
手术仍然是脊柱转移瘤的标准治疗方法。然而，术中出血难以控制对充分的手术切除构成了重大挑战，并影响手术效果。在本研究中，我们通过将纳米机器人整合到再生丝素蛋白纳米纤维水凝胶中，开发了一种负载凝血酶（Thr）的纳米机器人 - 水凝胶混合超结构。这种具有优异触变性的超结构在小鼠模型的脊柱手术前经皮注射并分散到具有易出血特征的肝细胞癌（HCC）脊柱转移瘤中。在近红外照射下，自主运动的纳米机器人穿透到深部脊柱肿瘤中，以可控方式释放Thr。Thr诱导的血栓形成有效地阻断肿瘤血管并减少出血，通过金纳米棒介导的光热疗法抑制肿瘤生长和术后复发。我们的微创治疗平台为HCC脊柱转移瘤提供了一种新的术前治疗策略，可有效控制术中出血和肿瘤生长，潜在地减少手术并发症并提高手术效果。
