Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Bioconjug Chem. 2021 Feb 17;32(2):367-375. doi: 10.1021/acs.bioconjchem.1c00002. Epub 2021 Jan 15.
The synthesis and anticancer cell activity of nitric oxide (NO)-releasing carbon quantum dots (CQDs) are described as potential theranostics. A series of secondary amine-modified CQDs were prepared using a hydrothermal method to modify β-cyclodextrin with hydroxyl and primary amine terminal functional groups. Subsequent reaction of the CQDs with NO gas under alkaline conditions yielded -diazeniumdiolate NO donor-modified CQDs with adjustable NO payloads (0.2-1.1 μmol/mg) and release kinetics (half-lives from 29 to 79 min) depending on the level of secondary amines and surface functional groups. The anticancer activity of the NO-releasing CQDs against Pa14c, A549, and SW480 cancer cell lines proved to be dependent on both NO payloads and surface functionalizations. Primary amine-modified CQDs with NO payloads ∼1.11 μmol/mg exhibited the greatest anticancer action. A fluorescence microscopy study demonstrated the utility of these NO-releasing CQDs as dual NO-releasing and bioimaging probes.
描述了一氧化氮（NO）释放碳量子点（CQDs）的合成及其抗癌细胞活性，可作为潜在的治疗方法。采用水热法，以具有羟基和伯胺端基官能团的β-环糊精为原料，制备了一系列仲胺修饰的 CQDs。随后，在碱性条件下，CQDs 与 NO 气体反应，生成 -二氮烯二酸盐 NO 供体修饰的 CQDs，其 NO 载药量（0.2-1.1 μmol/mg）和释放动力学（半衰期为 29-79 min）可根据仲胺和表面官能团的水平进行调节。NO 释放 CQDs 对 Pa14c、A549 和 SW480 癌细胞系的抗癌活性取决于 NO 载药量和表面官能化。NO 载药量约为 1.11 μmol/mg 的伯胺修饰 CQDs 表现出最大的抗癌作用。荧光显微镜研究表明，这些 NO 释放 CQDs 可用作双重 NO 释放和生物成像探针。
