Sabouri Leila, Farzin Ali, Kabiri Azadeh, Milan Peiman Brouki, Farahbakhsh Mojtaba, Mehdizadehkashi Abolfazl, Kajbafzadeh Abdolmohammad, Samadikuchaksaraei Ali, Yousefbeyk Fatemeh, Azami Mahmoud, Moghtadaei Mehdi
Cellular and Molecular Research Center, Iran University of Medical Sciences 1449614535, Tehran, Iran.
Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran 1449614535, Iran.
ACS Biomater Sci Eng. 2020 Nov 9;6(11):6285-6298. doi: 10.1021/acsbiomaterials.0c00881. Epub 2020 Oct 19.
The human amniotic membrane (HAM) has been viewed as a potential regenerative material for a wide variety of injured tissues because of its collagen-rich content. High degradability of HAM limits its wide practical application in bone tissue engineering. In this study, the natural matrix of the decellularized amniotic membrane was developed by the double diffusion method. The results confirmed a reduction of the amniotic membrane's degradability because of the deposition of calcium and phosphate ions during the double diffusion process. Real-time PCR results showed a high expression of osteogenesis-related genes from adipose-derived mesenchymal stem cells (ADMSCs) cultured on the surface of the developed mineralized amniotic membrane (MAM). Further experiments were conducted using an MAM preseeded with ADMSCs and a critical-size rat calvarial defect model. Histopathological results confirmed that the MAM + cell sample has excellent potential in bone regeneration.
由于富含胶原蛋白，人羊膜（HAM）被视为多种受损组织的潜在再生材料。HAM的高降解性限制了其在骨组织工程中的广泛实际应用。在本研究中，通过双扩散法制备了脱细胞羊膜的天然基质。结果证实，由于双扩散过程中钙和磷酸根离子的沉积，羊膜的降解性降低。实时PCR结果显示，在制备的矿化羊膜（MAM）表面培养的脂肪来源间充质干细胞（ADMSC）中，成骨相关基因高表达。使用预先接种ADMSC的MAM和临界大小的大鼠颅骨缺损模型进行了进一步实验。组织病理学结果证实，MAM+细胞样本在骨再生方面具有优异的潜力。
