Octarina Octarina, Munadziroh Elly, Razak Fathilah Abdul, Handharyani Ekowati, Surboyo Meircurius Dwi Condro
Department of Dental Material, Faculty of Dentistry, Universitas Trisakti, Jakarta 11440, Indonesia.
Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia.
Int J Biomater. 2024 Sep 14;2024:4053527. doi: 10.1155/2024/4053527. eCollection 2024.
Ridge preservation is an important technique for maintaining the dimensions of the alveolar bone following tooth extraction, which is crucial for successful tooth rehabilitation. The combination of bovine amniotic membrane and hydroxyapatite has shown promise as a scaffold material containing growth factors that can stimulate osteogenic-related factors such as bone morphogenetic protein 2 (BMP2), Runt-related transcription factor 2 (RUNX2), and osteocalcin. This stimulation leads to collagen production and osteoblast proliferation, resulting in new bone formation. In this study, bovine amniotic membrane-hydroxyapatite (BAM-HA) composites were prepared using three different ratios of bovine amniotic membrane and hydroxyapatite (2 : 3, 3 : 7, 7 : 13). Thirty rats had their first incisors extracted, and different types of BAM-HA were applied for ridge preservation. The control group received no treatment, while the positive control group was given xenograft. After 14 and 28 days, the animals were sacrificed, and immunohistochemical analysis was performed to evaluate the expression of BMP2, RUNX2, and osteocalcin. Additionally, a histological examination was conducted to analyse collagen thickness and osteoblast cell proliferation. The results demonstrated that the application of BAM-HA significantly increased collagen density, osteoblast cell proliferation, and the expression of BMP2, RUNX2, and osteoclacin compared to the control group ( < 0.05) on both days 14 and 28. Furthermore, increasing the hydroxyapatite content in the composite was found to enhance collagen thickness, osteoblast cell proliferation, and the expression of osteogenic-related factors. These preliminary findings suggest that the combination of BAM-HA can be used for ridge preservation to prevent further bone resorption following tooth extraction.
牙槽嵴保存是拔牙后维持牙槽骨尺寸的一项重要技术，这对于成功的牙齿修复至关重要。牛羊膜和羟基磷灰石的组合已显示出作为一种含有生长因子的支架材料的潜力，这些生长因子可以刺激骨形态发生蛋白2（BMP2）、 runt相关转录因子2（RUNX2）和骨钙素等成骨相关因子。这种刺激导致胶原蛋白生成和成骨细胞增殖，从而形成新骨。在本研究中，使用三种不同比例的牛羊膜和羟基磷灰石（2∶3、3∶7、7∶13）制备了牛羊膜 - 羟基磷灰石（BAM - HA）复合材料。30只大鼠的第一颗门牙被拔除，并应用不同类型的BAM - HA进行牙槽嵴保存。对照组未接受治疗，而阳性对照组给予异种移植物。在14天和28天后，处死动物并进行免疫组织化学分析以评估BMP2、RUNX2和骨钙素的表达。此外，进行了组织学检查以分析胶原厚度和成骨细胞增殖。结果表明，与对照组相比，在第14天和第28天，应用BAM - HA均显著增加了胶原密度、成骨细胞增殖以及BMP2、RUNX2和骨钙素的表达（<0.05）。此外，发现增加复合材料中羟基磷灰石的含量可增强胶原厚度、成骨细胞增殖以及成骨相关因子的表达。这些初步研究结果表明，BAM - HA组合可用于牙槽嵴保存，以防止拔牙后进一步的骨吸收。
