Ghasemi Maedeh, Sadeghimahalli Forouzan, Jamali Hassan, Yazdi Azadeh, Seyedi Moqadam Mohammad Reza
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
mmunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Avicenna J Phytomed. 2025 Jul-Aug;15(4):1379-1390. doi: 10.22038/ajp.2025.25486.
Chronic stress is a common and fundamental problem in human life all over the world which threatens the health. Stress-induced metabolic disorders are attenuated by natural honey feeding. So, we examined protective impact of thyme honey in the regulation of blood glucose via measuring the expression level of muscle GLUT4 protein in chronic unpredictable mild stressed (CUMS) male rats.
Six groups of adult male Wistar rats were designed in this study; control group that received water; unstressed groups that were treated with honey (0.2 and 2 g/kg/day) for 38 days; stressed group that received CUMS for 4 weeks; treated stressed groups that were gavaged by honey (0.2 and 2 g/kg/day) for 38 days (from 10 days before induction of stress until the end of stress period). A day after the experiment period (39 day), in non-fasting status, rats were sacrificed to measure glucose, insulin, irisin, lipid profile at serum level and GLUT4 protein content in muscle tissue via western blotting method.
Honey reduced hyperglycemia induced by CUMS, significantly increased serum irisin and non-significantly increased high-density lipoprotein cholesterol (HDL-c) which were decreased by CUMS, but did not affect other serum lipids and insulin. CUMS down-regulated GLUT4 protein level. Honey feeding (2 g/kg) in stressed rats interestingly increased level of GLUT4 protein and maintained it at a normal level.
Together, it may be concluded that honey administration protects glycemic control system from chronic stress-induced dysregulation via an increase in production of irisin and maintaining the GLUT4 protein levels.
慢性应激是全球人类生活中一个常见且基本的问题，威胁着健康。应激诱导的代谢紊乱可通过喂食天然蜂蜜得到缓解。因此，我们通过测量慢性不可预测轻度应激（CUMS）雄性大鼠肌肉葡萄糖转运蛋白4（GLUT4）蛋白的表达水平，研究了百里香蜂蜜在调节血糖方面的保护作用。
本研究设计了六组成年雄性Wistar大鼠；对照组给予水；未应激组用蜂蜜（0.2和2克/千克/天）处理38天；应激组接受4周的CUMS处理；应激处理组在应激诱导前10天开始至应激期结束，用蜂蜜（0.2和2克/千克/天）灌胃38天。实验期（第39天）后一天，在非空腹状态下，处死大鼠，通过蛋白质免疫印迹法测量血清中的葡萄糖、胰岛素、鸢尾素、血脂水平以及肌肉组织中GLUT4蛋白含量。
蜂蜜降低了CUMS诱导的高血糖，显著增加了血清鸢尾素，非显著增加了被CUMS降低的高密度脂蛋白胆固醇（HDL-c），但对其他血脂和胰岛素无影响。CUMS下调了GLUT4蛋白水平。有趣的是，给应激大鼠喂食蜂蜜（2克/千克）增加了GLUT4蛋白水平并将其维持在正常水平。
综上所述，可以得出结论，给予蜂蜜可通过增加鸢尾素的产生和维持GLUT4蛋白水平，保护血糖控制系统免受慢性应激诱导的失调。
