Department of Pharmacognosy and Molecular Basis of Phytotherapy, Centre for Preclinical Studies, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland; National University of Pharmacy, 53 Pushkinska Str., Kharkiv, 61002, Ukraine.
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Centre for Preclinical Studies, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
Food Chem. 2021 Jun 1;346:128929. doi: 10.1016/j.foodchem.2020.128929. Epub 2020 Dec 27.
Linden trees are a source of food products called lime flowers (Tiliae flos), traditionally used in the form of infusion for the treatment of feverish colds and coughs. Lime flowers should include flowers of Tilia cordata Mill, T.x europaea L., and T. platyphyllos Scop. or a mixture of these. The aim of current research was to establish a fast, sensitive HPTLC (high-performance thin-layer chromatography) method that would allow the differentiation of material obtained from five species of lime occurring in Europe. The fingerprints for distinguishing these species were established, as well as a key for identification based on a visual evaluation of chromatograms. The results obtained were also subjected to chemometric analyses. It was shown that each species contains characteristic compounds i.e. linarin that can be used for their identification. The method developed can, in theory, be introduced for the quality control or authentication of linden flowers on the European market.
椴树是一种被称为椴花（Tiliae flos）的食品来源，传统上以浸泡的形式用于治疗发热性感冒和咳嗽。椴花应包括毛果椴（Tilia cordata Mill）、欧洲椴（T.x europaea L.）和欧洲大叶椴（T. platyphyllos Scop.）的花，或这些花的混合物。目前研究的目的是建立一种快速、灵敏的 HPTLC（高效薄层色谱）方法，以区分来自欧洲的五种椴树的材料。建立了用于区分这些物种的指纹图谱，并基于色谱图的视觉评估建立了识别的关键。还对所得结果进行了化学计量学分析。结果表明，每个物种都含有可用于识别的特征化合物，即滨黎苷。该方法理论上可用于欧洲市场上椴花的质量控制或鉴定。
