Toker G, Aslan M, Yeşilada E, Memişoğlu M, Ito S
Department of Pharmacognosy, Eczacilik Fakültesi, Gazi Universitesi, Hipodrom, 06330 Ankara, Turkey.
J Pharm Biomed Anal. 2001 Aug;26(1):111-21. doi: 10.1016/s0731-7085(01)00351-x.
Lime flowers are used for various medicinal purposes in phytotherapy. Flavonoids, volatile oil and mucilage components are known as the active ingredients. In European Pharmacopoeia (EP), a simple thin-layer chromatographic (TLC) technique, which based on the analysis of the flavonoid composition was defined for the qualitative analysis of the drug. In this study, flavonoid composition in the flowers, bracts and leaves of the officinal species, Tilia platyphyllos were studied using a reversed-phase high performance liquid chromatographic (HPLC) technique, in order to develop a rapid, reliable and accurate method for quantitative analysis. The results were further compared with in those parts of two common species growing in Turkey, Tilia rubra and Tilia argentea. Results of the present study revealed that flavonoid composition of each lime species possesses a specific fingerprint HPLC chromatogram depending upon the parts used and evaluation of the data might be helpful in the quality assurance as well as determination of adulteration of the crude drug.
菩提树花在植物疗法中有多种药用用途。黄酮类化合物、挥发油和黏液成分被认为是其活性成分。在欧洲药典（EP）中，定义了一种基于黄酮类成分分析的简单薄层色谱（TLC）技术用于该药物的定性分析。在本研究中，使用反相高效液相色谱（HPLC）技术研究了药用植物阔叶椴的花、苞片和叶中的黄酮类成分，以开发一种快速、可靠且准确的定量分析方法。还将结果与在土耳其生长的两种常见物种——红椴和银叶椴的这些部位进行了比较。本研究结果表明，每种菩提树花的黄酮类成分具有特定的指纹HPLC色谱图，这取决于所使用的部位，并且数据评估可能有助于粗药物的质量保证以及掺假测定。
