Genthner F J, Chatterjee P, Barkay T, Bourquin A W
Environmental Research Laboratory, U.S. Environmental Protection Agency, Gulf Breeze, Florida 32561.
Appl Environ Microbiol. 1988 Jan;54(1):115-7. doi: 10.1128/aem.54.1.115-117.1988.
A total of 68 gram-negative freshwater bacterial isolates were screened for their ability to receive and express plasmids from Pseudomonas aeruginosa donors. The plate mating technique identified 26 of the isolates as recipient active for the self-transmissible wide-host-range plasmid R68; 10 were recipient active by R68 mobilization for the wide-host-range plasmid cloning vector R1162. Frequencies of transfer were compared by using three conjugal transfer procedures: broth, plate, and filter mating. For every recipient tested, a solid environment was superior to a liquid environment for transfer. The broth mating technique failed to demonstrate R68 transfer in 63% of the recipient-active isolates. Filter mating, in general, yielded the highest transfer frequencies. The more-rapid plate mating procedure, however, was just as sensitive for testing the capacity of natural isolates to participate in conjugal plasmid transfer.
总共筛选了68株革兰氏阴性淡水细菌分离株，以检测它们从铜绿假单胞菌供体接收和表达质粒的能力。平板交配技术鉴定出26株分离株对自我传递的广宿主范围质粒R68具有受体活性；10株通过R68动员对广宿主范围质粒克隆载体R1162具有受体活性。通过使用三种接合转移程序（肉汤、平板和滤膜交配）比较转移频率。对于每一个测试的受体，固体环境在转移方面优于液体环境。肉汤交配技术在63%的受体活性分离株中未能证明R68转移。一般来说，滤膜交配产生的转移频率最高。然而，更快速的平板交配程序在测试天然分离株参与接合性质粒转移的能力方面同样敏感。
