Madsen E L, Francis A J, Bollag J M
Department of Agronomy, Pennsylvania State University, University Park 16802.
Appl Environ Microbiol. 1988 Jan;54(1):74-8. doi: 10.1128/aem.54.1.74-78.1988.
The influence of physiological and environmental factors on the accumulation of oxindole during anaerobic indole metabolism was investigated by high-performance liquid chromatography. Under methanogenic conditions, indole was temporarily converted to oxindole in stoichiometric amounts in media inoculated with three freshwater sediments and an organic soil. In media inoculated with methanogenic sewage sludge, the modest amounts of oxindole detected at 35 degrees C reached higher concentrations and persisted longer when the incubation temperature was decreased from 35 to 15 degrees C. Also, decreasing the concentration of sewage sludge used as an inoculum from 50 to 1% caused an increase in the accumulation of oxindole from 10 to 75% of the indole added. Under denitrifying conditions, regardless of the concentration or source of the inoculum, oxindole appeared in trace amounts but did not accumulate during indole metabolism. In addition, denitrifying consortia which previously metabolized indole degraded oxindole with no lag period. Our data suggest that oxindole accumulation under methanogenic, but not under denitrifying conditions is caused by differences between relative rates of oxindole production and destruction.
通过高效液相色谱法研究了生理和环境因素对厌氧吲哚代谢过程中氧化吲哚积累的影响。在产甲烷条件下，在接种了三种淡水沉积物和一种有机土壤的培养基中，吲哚以化学计量的量暂时转化为氧化吲哚。在接种了产甲烷污水污泥的培养基中，当培养温度从35℃降至15℃时，在35℃检测到的少量氧化吲哚达到了更高的浓度且持续时间更长。此外，将用作接种物的污水污泥浓度从50%降至1%，导致氧化吲哚的积累量从添加吲哚量的10%增加到75%。在反硝化条件下，无论接种物的浓度或来源如何，氧化吲哚以痕量出现，但在吲哚代谢过程中不积累。此外，先前代谢吲哚的反硝化菌群降解氧化吲哚没有滞后期。我们的数据表明，产甲烷条件下而非反硝化条件下氧化吲哚的积累是由氧化吲哚产生和破坏的相对速率差异引起的。
