人绒毛膜促性腺激素酶联受体分析方法（ERA）的开发。

Development of an enzyme-linked receptor assay (ERA) for hCG.

作者信息

Hashimoto M, Kawaoi A

出版信息

Endocrinol Jpn. 1977 Jun;24(3):307-12. doi: 10.1507/endocrj1954.24.307.

DOI:10.1507/endocrj1954.24.307

Abstract

An enzyme-linked receptor assay (ERA) for hCG was developed using horseradish peroxidase. The 1,500 g pellets, interstitial cell fraction, and solubilized homogenate from the rat testis linked to tanned sheep red blood cells (SRBC) were used as the binding fractions for hCG. In these ERA systems, the limit of detection for hCG was 30 IU/l (3-12 mIU/tube), which was almost equal to that of radio receptor assay (RRA). The ERA using SRBC linked solubilized receptor fraction showed the most satisfactory result in accuracy, reproducibility and easiness.

摘要

使用辣根过氧化物酶开发了一种用于检测人绒毛膜促性腺激素（hCG）的酶联受体测定法（ERA）。将来自大鼠睾丸的1500g沉淀、间质细胞部分以及与鞣酸处理的绵羊红细胞（SRBC）相连的溶解匀浆用作hCG的结合部分。在这些ERA系统中，hCG的检测限为30IU/L（3 - 12mIU/管），这几乎与放射受体测定法（RRA）的检测限相等。使用与SRBC相连的溶解受体部分的ERA在准确性、重现性和简便性方面显示出最令人满意的结果。