人绒毛膜促性腺激素的同步竞争性酶免疫测定法。
Simultaneous competitive enzyme immunoassay for human chorionic gonadotropin.
作者信息
Hamada K, Tomoda S, Sugawa T, Takahashi K P
出版信息
Endocrinol Jpn. 1978 Oct;25(5):515-7. doi: 10.1507/endocrj1954.25.515.
A simultaneous competitive enzyme immunoassay (SICEIA) for hCG was developed using beta-D-galactosidase (beta-Gal) as a labelled enzyme and anti-hCG antibody coated sheep red blood cells (SRBC) as a solid phase. In this report, a new coupling agent, MCAE, was used to couple beta-Gal with hCG. The sensitivity was improved to the degree of 2.5 mIU/ml, equal to that of RIA. The present procedure was safer and rapider than RIA. The value of hCG in urine by our procedure had good correlation with that by RIA.
建立了一种以β-D-半乳糖苷酶（β-Gal）为标记酶、抗人绒毛膜促性腺激素（hCG）抗体包被的绵羊红细胞（SRBC）为固相的hCG同步竞争酶免疫分析方法（SICEIA）。在本报告中，使用了一种新型偶联剂MCAE将β-Gal与hCG偶联。灵敏度提高到2.5 mIU/ml，与放射免疫分析（RIA）相当。本方法比RIA更安全、更快速。用我们的方法检测尿液中hCG的值与RIA法检测的值具有良好的相关性。
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