Сardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.
Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
Bull Exp Biol Med. 2021 Jan;170(3):368-370. doi: 10.1007/s10517-021-05069-8. Epub 2021 Jan 16.
We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old (n=8) and 15-month-old (n=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals, it was significantly (р<0.05) lower: 3.57 (3.50; 3.62). Pyrene probe eximerization indices for regions of protein-lipid and lipid-lipid interactions in mitochondria of 2-month-old animals were significantly lower than in the group of 15-month-old rats, which indicated reduced microviscosity of the lipid environment of proteins. Thus, the decrease in RC of mitochondria from the left ventricle of 15-month-old animals and the increase in the microviscosity coefficients of their membranes indicate age-related changes in the structural and functional activity of mitochondria.
我们研究了 2 个月大（n=8）和 15 个月大（n=8）大鼠左心室心肌中线粒体膜的呼吸活性和微观粘度。在存在 ADP 和利用 ADP 后，通过计算氧吸收速率的比值来评估底物磷酸化过程中的呼吸控制（RC）。在 2 个月大的大鼠中，RC 为 4.66（4.56；4.71）；而在 15 个月大的动物中，RC 显著降低（р<0.05）：3.57（3.50；3.62）。2 个月大动物的芘探针激基缔合物指数表明，线粒体中蛋白质-脂质和脂质-脂质相互作用区域的微观粘度显著低于 15 个月大的大鼠组，这表明蛋白质脂质环境的微观粘度降低。因此，15 个月大动物左心室线粒体 RC 的降低以及其膜的微观粘度系数的增加表明，线粒体的结构和功能活性随年龄而变化。
