Research Center of Family Health and Human Reproduction Problems, Irkutsk, Russia.
Bull Exp Biol Med. 2021 Jan;170(3):321-325. doi: 10.1007/s10517-021-05060-3. Epub 2021 Jan 16.
Opportunistic microorganisms in the gut biocenosis were studied in adolescents with normal body weight and obesity (patients consulted at the Clinical Department of Research Center of Family Health and Human Reproduction Problems). The biological material was studied by standard bacteriological methods, representatives of Enterobacteriaceae family were also characterized using metagenomic sequencing of V3-V4 variable regions of 16S gene rRNA. Gut microbiota of obese adolescents was unbalanced and was characterized by low levels of bifido- and lactoflora representatives, a spectrum of E. coli associations, and high prevalence of opportunistic microorganisms and their associations. Representatives of Enterobacteriaceae family were most often found in the gut microbiota of obese adolescents.
研究了正常体重和肥胖青少年（在家庭健康和人类生殖问题研究中心临床科就诊的患者）肠道生物群落中的机会性微生物。使用标准细菌学方法研究生物材料，还使用 16S 基因 rRNA V3-V4 可变区的宏基因组测序对肠杆菌科家族的代表进行了表征。肥胖青少年的肠道微生物群失衡，双歧杆菌和乳杆菌代表物水平低，大肠杆菌组合谱广泛，机会性微生物及其组合的流行率高。肠杆菌科家族的代表最常存在于肥胖青少年的肠道微生物群中。
