19F-核磁共振：生物系统中[O2]和pH的测量
19F-nuclear magnetic resonance: measurements of [O2] and pH in biological systems.
作者信息
Taylor J, Deutsch C
机构信息
Department of Physiology, Univesity of Pennsylvania School of Medicine, Philadelphia 19104-6085.
出版信息
Biophys J. 1988 Feb;53(2):227-33. doi: 10.1016/S0006-3495(88)83084-4.
19F-nuclear magnetic resonance (NMR) has been used to determine both intracellular pH and oxygen concentrations in cell suspensions. Oxygen concentrations in Paracoccus denitrificans and insulinoma cells, RINm5F, in the NMR probe can be monitored directly by 1/T1 measurements of perfluorotripropylamine (FTPA)/lecithin emulsion added to the suspensions. With FTPA oxygen monitoring, we investigated the relative aeration capabilities of two types of NMR chambers. Both normal and transformed eucaryotic cells can be maintained in either chamber for at least 1-2 h at cytocrits of up to 20-25%, with 30% oxygen saturation and cell viabilities of 90-95%. Similar concentrations of procaryotes were maintained aerobic with high FTPA concentrations in the more efficient of the two NMR chambers. A new precursor molecule for the 19F-NMR pH indicator difluoromethylalanine, the para-chlorophenyl ester, has been tested and used in RINm5F cells and P. denitrificans, neither of which hydrolyzes methyl esters.
19F-核磁共振（NMR）已被用于测定细胞悬液中的细胞内pH值和氧浓度。通过对添加到悬液中的全氟三丙胺（FTPA）/卵磷脂乳液进行1/T1测量，可以直接监测NMR探头中反硝化副球菌和胰岛素瘤细胞RINm5F中的氧浓度。利用FTPA氧监测，我们研究了两种类型NMR腔室的相对通气能力。正常和转化的真核细胞在两种腔室中均可在血细胞比容高达20%-25%的情况下维持至少1-2小时，氧饱和度为30%，细胞活力为90%-95%。在两个NMR腔室中效率更高的那个腔室中，较高浓度的FTPA可使类似浓度的原核生物保持有氧状态。19F-NMR pH指示剂二氟甲基丙氨酸的一种新前体分子对氯苯酯，已在RINm5F细胞和反硝化副球菌中进行了测试和应用，这两种细胞均不会水解甲酯。
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