Department of Biochemistry and Molecular Biology and Center for Eukaryotic Gene Regulation, Pennsylvania State University, University Park, Pennsylvania, USA.
Department of Biochemistry and Molecular Biology and Center for Eukaryotic Gene Regulation, Pennsylvania State University, University Park, Pennsylvania, USA; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
J Biol Chem. 2020 Dec 18;295(51):17411-17412. doi: 10.1074/jbc.H120.016607.
Defining discontinuous antigenic epitopes remains a substantial challenge, as exemplified by the case of lipid transfer polyproteins, which are common pollen allergens. Hydrogen/deuterium exchange monitored by NMR can be used to map epitopes onto folded protein surfaces, but only if the complex rapidly dissociates. Modifying the standard NMR-exchange measurement to detect substoichiometric complexes overcomes this time scale limitation and provides new insights into recognition of lipid transfer polyprotein by antibodies. In the future, this new and exciting development should see broad application to a range of tight macromolecular interactions.
定义不连续的抗原表位仍然是一个巨大的挑战，脂质转移多蛋白就是一个很好的例子，它是常见的花粉过敏原。通过 NMR 监测的氢/氘交换可用于将表位映射到折叠蛋白质表面，但前提是该复合物快速解离。通过修改标准的 NMR 交换测量来检测亚化学计量复合物，克服了这个时间尺度限制，并为抗体识别脂质转移多蛋白提供了新的见解。在未来，这一新的令人兴奋的发展应该会广泛应用于一系列紧密的大分子相互作用。
