National Institute of Metrology, Beijing, 100029, China.
College of Chemistry, Beijing University of Chemical Technology, Beijing, 100029, China.
Anal Bioanal Chem. 2021 Apr;413(9):2345-2359. doi: 10.1007/s00216-020-03091-9. Epub 2021 Jan 6.
With the development of biomedical technology, epitope mapping of proteins has become critical for developing and evaluating new protein drugs. The application of hydrogen-deuterium exchange for protein epitope mapping holds great potential. Although several reviews addressed the hydrogen-deuterium exchange, to date, only a few systematic reviews have focused on epitope mapping using this technology. Here, we introduce the basic principles, development history, and review research progress in hydrogen-deuterium exchange epitope mapping technology and discuss its advantages. We summarize the main hurdles in applying hydrogen-deuterium exchange epitope mapping technology, combined with relevant examples to provide specific solutions. We describe the epitope mapping of virus assemblies, disease-associated proteins, and polyclonal antibodies as examples of pattern introduction. Finally, we discuss the outlook of hydrogen-deuterium exchange epitope mapping technology. This review will help researchers studying protein epitopes to gain a more comprehensive understanding of this technology.
随着生物医学技术的发展，蛋白质表位作图对于开发和评估新型蛋白质药物至关重要。氢氘交换在蛋白质表位作图中的应用具有巨大的潜力。尽管有几篇综述讨论了氢氘交换，但迄今为止，只有少数系统综述专注于使用该技术进行表位作图。在这里，我们介绍了氢氘交换表位作图技术的基本原理、发展历史和综述研究进展，并讨论了它的优势。我们总结了应用氢氘交换表位作图技术的主要障碍，并结合相关实例提供了具体的解决方案。我们以病毒组装体、疾病相关蛋白和多克隆抗体的表位作图为例进行模式介绍。最后，我们讨论了氢氘交换表位作图技术的展望。本综述将帮助研究蛋白质表位的研究人员更全面地了解这项技术。
