Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India; email:
Departments of Molecular Genetics, Biochemistry, and Chemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada; email:
Annu Rev Biophys. 2019 May 6;48:297-319. doi: 10.1146/annurev-biophys-052118-115647. Epub 2019 Mar 22.
Biological molecules are often highly dynamic, and this flexibility can be critical for function. The large range of sampled timescales and the fact that many of the conformers that are continually explored are only transiently formed and sparsely populated challenge current biophysical approaches. Solution nuclear magnetic resonance (NMR) spectroscopy has emerged as a powerful method for characterizing biomolecular dynamics in detail, even in cases where excursions involve short-lived states. Here, we briefly review a number of NMR experiments for studies of biomolecular dynamics on the microsecond-to-second timescale and focus on applications to protein and nucleic acid systems that clearly illustrate the functional relevance of motion in both health and disease.
生物分子通常具有高度的动态性，这种灵活性对于其功能至关重要。当前的生物物理方法面临的挑战是，生物分子的采样时间尺度范围很广，而且许多不断被探索的构象只是短暂形成的，并且稀疏存在。溶液核磁共振（NMR）光谱学已成为一种强大的方法，可以详细描述生物分子动力学，即使在涉及短暂状态的情况下也是如此。在这里，我们简要回顾了一些用于研究微秒至秒时间尺度上生物分子动力学的 NMR 实验，并重点介绍了蛋白质和核酸系统的应用，这些应用清楚地说明了运动在健康和疾病中的功能相关性。
