Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Slovakia.
Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Slovakia; Research Institute for Child Psychology and Pathopsychology, Bratislava, Slovakia.
Int J Psychophysiol. 2021 Mar;161:86-90. doi: 10.1016/j.ijpsycho.2021.01.007. Epub 2021 Jan 14.
The effect of chronic stress on oxidative stress (OS) is commonly discussed while the effect of acute stress situation is not fully examined yet. The present study was aimed to analyse whether acute psychosocial strain causes changes in OS and antioxidant status. Unstimulated saliva was collected from 46 healthy prepubertal children during the control and stress day. On the stress day, collection was performed before and after a stress situation induced by the Trier social stress test. Saliva collection during the control day imitated the stress day without the stress strain. Samples were used for analysis of lipid peroxidation, thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGEs) and markers of antioxidant status, total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP). On the stress day, increased level of FRAP was observed in the second saliva collection in comparison with the first collection. Within the same day, no significant changes in the levels of TBARS, AGEs and TAC were observed in samples taken before and after stress strain. Significantly higher levels of TBARS were observed on stress day in comparison to control day. In summary, acute psychosocial stress caused increase of FRAP during the stress day. TBARS did not increase during the stress day in the second sample but it was higher compared to the control day. None of the interactions with gender were statistically significant. It appears the short-term exposure to stress could potentially stimulate antioxidant activity.
慢性应激对氧化应激（OS）的影响经常被讨论，而急性应激情况的影响尚未完全研究。本研究旨在分析急性心理社会应激是否会引起 OS 和抗氧化状态的变化。在对照和应激日，从 46 名健康青春期前儿童中采集未刺激的唾液。在应激日，在特里尔社会应激测试引起的应激情况之前和之后进行采集。对照日的唾液采集模仿了没有应激的应激日。样本用于分析脂质过氧化、硫代巴比妥酸反应物质（TBARS）、晚期糖基化终产物（AGEs）和抗氧化状态标志物，总抗氧化能力（TAC）和铁还原抗氧化能力（FRAP）。在应激日，与第一次采集相比，第二次采集的 FRAP 水平升高。在同一天内，应激前和应激后采集的样本中 TBARS、AGEs 和 TAC 的水平没有显著变化。与对照日相比，应激日的 TBARS 水平显著升高。总之，急性心理社会应激导致应激日 FRAP 升高。TBARS 在第二次采集时在应激日没有增加，但与对照日相比更高。性别与任何指标之间均无显著交互作用。短期暴露于应激可能会刺激抗氧化活性。
