Cai Hanyang, Huang Youmei, Chen Fangqian, Liu Liping, Chai Mengnan, Zhang Man, Yan Maokai, Aslam Mohammad, He Qing, Qin Yuan
College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Guangxi Key Lab of Sugarcane Biology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning, 530004, China.
New Phytol. 2021 Apr;230(2):737-756. doi: 10.1111/nph.17200. Epub 2021 Feb 18.
The signaling pathway mediated by the receptor-like kinase ERECTA (ER) plays important roles in plant immune responses, but the underlying mechanism is unclear. Genetic interactions between ER signaling and the chromatin remodeling complex SWR1 in the control of plant immune responses were studied. Electrophoretic mobility shift assay and yeast one-hybrid analysis were applied to identify ER-WRKY33 downstream components. Chromatin immunoprecipitation analyses were further investigated. In this study, we show that the chromatin remodeling complex SWR1 enhances resistance to the white mold fungus Sclerotinia sclerotiorum in Arabidopsis thaliana via a process mediated by ER signaling. We identify a series of WRKY33 target YODA DOWNSTREAM (YDD) genes and demonstrate that SWR1 and ER signaling are required to enrich H2A.Z histone variant and H3K4me3 histone modification at YDDs and the binding of WRKY33 to YDD promoters upon S. sclerotiorum infection. We also reveal that the binding of WRKY33 to YDD promoters in turn promotes the enrichment of H2A.Z and H3K4me3 at YDD genes, thereby forming a positive regulatory loop to activate YDDs expression. Our study reveals how H2A.Z, H3K4me3 and ER signaling mutually regulate YDDs gene expression upon pathogen infection, highlighting the critical role of chromatin structure in ER-signaling-mediated plant immune responses.
类受体激酶ERECTA（ER）介导的信号通路在植物免疫反应中发挥重要作用，但其潜在机制尚不清楚。我们研究了ER信号与染色质重塑复合体SWR1在植物免疫反应调控中的遗传相互作用。应用电泳迁移率变动分析和酵母单杂交分析来鉴定ER-WRKY33的下游组分。并进一步开展了染色质免疫沉淀分析。在本研究中，我们发现染色质重塑复合体SWR1通过ER信号介导的过程增强了拟南芥对核盘菌的抗性。我们鉴定出一系列WRKY33靶标YODA下游（YDD）基因，并证明在核盘菌感染后，SWR1和ER信号是在YDDs处富集H2A.Z组蛋白变体和H3K4me3组蛋白修饰以及WRKY33与YDD启动子结合所必需的。我们还揭示了WRKY33与YDD启动子的结合反过来促进了YDD基因处H2A.Z和H3K4me3的富集，从而形成一个正调控环来激活YDDs的表达。我们的研究揭示了病原体感染时H2A.Z、H3K4me3和ER信号如何相互调节YDDs基因表达，突出了染色质结构在ER信号介导的植物免疫反应中的关键作用。
