Huang Youmei, Xi Xinpeng, Chai Mengnan, Ma Suzhuo, Su Han, Liu Kaichuang, Wang Fengjiao, Zhu Wenhui, Liu Yanhui, Qin Yuan, Cai Hanyang
Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Science, Longyan University, Longyan 364012, China.
Plants (Basel). 2023 Feb 19;12(4):940. doi: 10.3390/plants12040940.
Reactive oxygen species (ROS), a type of oxygen monoelectronic reduction product, play integral roles in root growth and development. The epigenetic mechanism plays a critical role in gene transcription and expression; however, its regulation of ROS metabolism in root development is still limited. We found that the chromatin remodeling complex SWR1 regulates root length and lateral root formation in . Our transcriptome results and gene ontology (GO) enrichment analysis showed that the oxidoreductase activity-related genes significantly changed in mutants for the SWR1 complex components, such as and , and histone variant H2A.Z triple mutant The three encoding genes in are the three H2A.Z variants , , and . Histochemical assays revealed that the SWR1 complex affects ROS accumulation in roots. Furthermore, chromatin immunoprecipitation quantitative real-time PCR (ChIP-qPCR) analysis showed that the reduced H2A.Z deposition in oxidoreductase activity-related genes caused ROS to accumulate in , , and . H2A.Z deposition-deficient mutants decreased after the trimethylation of lysine 4 on histone H3 (H3K4me3) modifications and RNA polymerase II (Pol II) enrichment, and increased after the trimethylation of lysine 27 on histone H3 (H3K27me3) modifications, which may account for the expression change in oxidoreductase activity-related genes. In summary, our results revealed that the chromatin complex SWR1 regulates ROS accumulation in root development, highlighting the critical role of epigenetic mechanisms.
活性氧（ROS）是一种单电子还原的氧产物，在根系生长发育中发挥着不可或缺的作用。表观遗传机制在基因转录和表达中起关键作用；然而，其在根系发育中对ROS代谢的调控作用仍较为有限。我们发现染色质重塑复合物SWR1在[具体植物]中调节根长和侧根形成。我们的转录组结果和基因本体（GO）富集分析表明，在SWR1复合物组分的突变体中，如[具体组分1]和[具体组分2]，以及组蛋白变体H2A.Z三突变体中，与氧化还原酶活性相关的基因发生了显著变化。[具体植物]中的三个编码基因是三个H2A.Z变体[变体1]、[变体2]和[变体3]。组织化学分析表明，SWR1复合物影响根系中ROS的积累。此外，染色质免疫沉淀定量实时PCR（ChIP-qPCR）分析表明，氧化还原酶活性相关基因中H2A.Z沉积减少导致ROS在[具体植物1]、[具体植物2]和[具体植物3]中积累。组蛋白H3赖氨酸4三甲基化（H3K4me3）修饰和RNA聚合酶II（Pol II）富集后，H2A.Z沉积缺陷型突变体减少，而组蛋白H3赖氨酸27三甲基化（H3K27me3）修饰后增加，这可能解释了氧化还原酶活性相关基因的表达变化。总之，我们的结果表明染色质复合物SWR1在根系发育中调节ROS积累，突出了表观遗传机制 的关键作用。
