Department of Oncology, 970 Hospital of the PLA Joint Logistic Support Force, Yantai, China.
J BUON. 2020 Nov-Dec;25(6):2552-2561.
Breast cancer is known as the second frequent cancer in the world, even as the most common cancer among women. This study aimed to explore the correlation of CXCL13/CXCR5 expression with clinical characteristics in breast cancer and evaluate their potential to be used as biomarkers in diagnosis and prognosis of this disease.
A total of 133 female patients diagnosed with breast cancer were collected. The expression of CXCL13 and CXCR5 mRNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining.
The expression of CXCL13 and CXCR5 was significantly higher in breast cancer tissue than in normal breast tissues, with a high correlation coefficient of 0.9973. Positive cell numbers and positive expression rates of CXCL13 and CXCR5 in cancer breast tissue were much higher than those in normal breast tissue, and raised with increase of cancer stage. The high expression of CXCL13 and CXCR5 in breast cancer tissue was notably associated with lymph node metastasis, distant metastasis, disease stage, but not with age, Her-2 status, histological type or tumor size. Immunohistochemistry analysis showed that the positive expression of CXCL13 and CXCR5 was related with cancer stage, Also, positive expression of CXCL13 was correlated with positive expression of CXCR5. Patient age, Her-2 status, tumor size, histological type, and lymph node metastasis were independent factors for the 5-year survival rate of breast cancer patients, whereas the 5-year survival was correlated with distant metastasis and expression of CXCL13 and CXCR5.
These results suggested that CXCL13 and CXCR5 expressions could act as potential biomarkers for breast cancer diagnosis and prognosis.
乳腺癌是世界上第二常见的癌症，甚至是女性中最常见的癌症。本研究旨在探讨 CXCL13/CXCR5 表达与乳腺癌临床特征的相关性，并评估其作为该疾病诊断和预后标志物的潜力。
收集了 133 名女性乳腺癌患者。采用实时定量聚合酶链反应（qRT-PCR）和免疫组织化学染色分析 CXCL13 和 CXCR5 mRNA 的表达。
乳腺癌组织中 CXCL13 和 CXCR5 的表达明显高于正常乳腺组织，相关系数为 0.9973。乳腺癌组织中 CXCL13 和 CXCR5 的阳性细胞数和阳性表达率明显高于正常乳腺组织，且随着癌症分期的升高而升高。乳腺癌组织中 CXCL13 和 CXCR5 的高表达与淋巴结转移、远处转移、疾病分期显著相关，与年龄、Her-2 状态、组织学类型或肿瘤大小无关。免疫组织化学分析显示，CXCL13 和 CXCR5 的阳性表达与癌症分期有关，而且，CXCL13 的阳性表达与 CXCR5 的阳性表达相关。患者年龄、Her-2 状态、肿瘤大小、组织学类型和淋巴结转移是乳腺癌患者 5 年生存率的独立因素，而 5 年生存率与远处转移和 CXCL13 和 CXCR5 的表达相关。
这些结果表明，CXCL13 和 CXCR5 的表达可以作为乳腺癌诊断和预后的潜在标志物。
