miR-21-3p 在胃癌中的预后潜能。

Prognostic potential of miR-21-3p in gastric cancer.

机构信息

Department of the Second Clinic of Internal Medicine, The 943 Hospital of the Joint Logistics Support Unit of the Chinese People's Liberation Army, Wuwei, China.

出版信息

J BUON. 2020 Nov-Dec;25(6):2678-2682.

PMID:33455113

Abstract

PURPOSE

This study aimed to clarify the role of microRNA (miR)-21-3p in regulating progression and prognosis in gastric cancer (GC).

METHODS

One hundred patients with primary GC were included in this study. Their primary GC tissues and paracancer normal mucosa were collected for detecting miR-21-3p levels. Receiver operating characteristic (ROC) curves were depicted for analyzing the predictive ability of miR-21-3p in GC. Subgroup analyses were conducted based on tumor size, lymph node metastasis status and TNM staging in GC patients. All GC patients were followed up for 5 years, and survival analysis was conducted using Kaplan-Meier method with log-rank test. Univariate and multivariate Cox regression analyses were performed for exploring potential prognostic factors for GC.

RESULTS

MiR-21-3p was highly expressed in GC tissues. Subgroup analyses were conducted based on tumor size, lymph node metastasis status and tumor staging. Subgroup analyses showed higher level of miR-21-3p in GC tissues collected from patients with large tumor size, lymph node metastasis or advanced TNM staging. ROC curves confirmed the diagnostic potential of miR-21-3p in GC. In addition, Kaplan-Meier and log-rank test revealed lower progression-free survival (PFS) and overall survival (OS) in GC patients overexpressing miR-21-3p. Tumor size, lymph node metastasis, TNM staging and miR-21-3p level were independent risk factors for the prognosis of GC.

CONCLUSIONS

MiR-21-3p is upregulated in GC samples, which is closely related to GC progression. MiR-21-3p can be used to predict the prognosis of GC.

摘要

目的

本研究旨在阐明 microRNA（miR）-21-3p 在调控胃癌（GC）进展和预后中的作用。

方法

本研究纳入了 100 例原发性 GC 患者。收集其原发性 GC 组织和癌旁正常黏膜组织，检测 miR-21-3p 水平。绘制受试者工作特征（ROC）曲线，分析 miR-21-3p 对 GC 的预测能力。根据 GC 患者的肿瘤大小、淋巴结转移状态和 TNM 分期进行亚组分析。对所有 GC 患者进行 5 年随访，采用 Kaplan-Meier 法和对数秩检验进行生存分析。采用单因素和多因素 Cox 回归分析探讨 GC 的潜在预后因素。

结果

miR-21-3p 在 GC 组织中高表达。根据肿瘤大小、淋巴结转移状态和肿瘤分期进行亚组分析。亚组分析显示，肿瘤体积较大、有淋巴结转移或 TNM 分期较晚的 GC 患者组织中 miR-21-3p 水平较高。ROC 曲线证实了 miR-21-3p 在 GC 中的诊断潜力。此外，Kaplan-Meier 和对数秩检验显示，miR-21-3p 高表达的 GC 患者无进展生存期（PFS）和总生存期（OS）较低。肿瘤大小、淋巴结转移、TNM 分期和 miR-21-3p 水平是 GC 预后的独立危险因素。