Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China.
J Clin Lab Anal. 2022 Jun;36(6):e24411. doi: 10.1002/jcla.24411. Epub 2022 Apr 21.
To find a useful disease marker for early diagnosis of gastric cancer, we tried to explore the expression of serum miR-181, miR-652, and carbohydrate antigen 72-4 (CA72-4).
According to clinical pathologic stages, 112 patients with gastric cancer were divided into early gastric cancer group (n = 60) and advanced gastric cancer group (n = 52), stage I-II (n = 65), and stage III-IV (n = 47). Another 50 cases of gastric benign lesions and 40 healthy controls were also selected. Real-time quantitative PCR together with chemiluminescence were applied to detect expression levels. ROC curve was applied to judge their diagnostic efficiency. Pearson's correlation analysis was put into use to investigate the relevance of three indicators.
Compared with benign lesions group and control group, significantly higher expression levels were found in patients of gastric cancer (all p < 0.001). Similarly, compared with early gastric cancer group, significantly higher expression levels were found in advanced gastric cancer group (all p < 0.001). The same result was also found in stage III-IV (all p < 0.001). The best cutoff values were 0.93, 2.38, and 16.94 U/ml, respectively. The area under the curve (0.917, 95%CI: 0.856-0.975) of the three combined diagnosis of early gastric cancer was the largest, and its sensitivity and specificity were 92.5% and 86.8%. And miR-181 and miR-652 were positively correlated with CA72-4 (r = 0.772, p < 0.001, r = 0.853, p < 0.001).
Serum miR-181, miR-652, and CA72-4 are closely linked to the occurrence and development of gastric cancer. Combination of three indicators has diagnostic value for early gastric cancer.
为了寻找对胃癌早期诊断有价值的疾病标志物，我们尝试探讨血清 miR-181、miR-652 和糖链抗原 72-4（CA72-4）的表达情况。
根据临床病理分期，将 112 例胃癌患者分为早期胃癌组（n=60）和进展期胃癌组（n=52）、Ⅰ-Ⅱ期（n=65）和Ⅲ-Ⅳ期（n=47）。另选取 50 例胃良性病变患者和 40 例健康对照者。应用实时定量 PCR 联合化学发光法检测其表达水平，应用 ROC 曲线判断其诊断效能，采用 Pearson 相关分析探讨 3 项指标的相关性。
与良性病变组和对照组相比，胃癌患者的表达水平明显升高（均 P<0.001）。同样，与早期胃癌组相比，进展期胃癌组的表达水平明显升高（均 P<0.001）。Ⅲ-Ⅳ期也存在同样的结果（均 P<0.001）。最佳截断值分别为 0.93、2.38 和 16.94 U/ml。3 项联合诊断早期胃癌的曲线下面积（AUC）最大，为 0.917（95%CI：0.856-0.975），其灵敏度和特异度分别为 92.5%和 86.8%。miR-181 和 miR-652 与 CA72-4 呈正相关（r=0.772，P<0.001，r=0.853，P<0.001）。
血清 miR-181、miR-652 和 CA72-4 与胃癌的发生发展密切相关，3 项指标联合检测对早期胃癌具有诊断价值。
