Jacques Erik, Hosoyama Katsuhiro, Biniam Brook, Eren Cimenci Cagla, Sedlakova Veronika, Steeves Alexander J, Variola Fabio, Davis Darryl R, Stewart Duncan J, Suuronen Erik J, Alarcon Emilio I
Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Ottawa, Ontario K1Y4W7, Canada.
Department of Cellular & Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ontario K1H8M5, Canada.
ACS Biomater Sci Eng. 2020 Aug 10;6(8):4614-4622. doi: 10.1021/acsbiomaterials.0c00245. Epub 2020 Jun 22.
As cell therapies emerged, it was quickly realized that pro-regenerative cells directly injected into injured tissue struggled within the inflammatory microenvironment. By using microencapsulation, i.e., encapsulating cells within polymeric biomaterials, they are henceforth protected from the harmful extracellular cues, while still being able to receive oxygen and nutrients and release secreted factors. Previous work showed that stem cells encapsulated within a biologically inert material (agarose) were able to significantly improve the function of the infarcted mouse heart. With the aim of using more bioresponsive microcapsules, we sought to develop an enzymatically degradable, type I collagen-based microcapsule for the intramyocardial delivery of bone marrow-derived mesenchymal stromal cells in a murine model of myocardial infarction.
随着细胞疗法的出现，人们很快意识到，直接注射到受损组织中的促再生细胞在炎症微环境中难以存活。通过微囊化，即将细胞包裹在聚合物生物材料中，细胞从此受到保护，免受有害的细胞外信号的影响，同时仍能够接收氧气和营养物质并释放分泌因子。先前的研究表明，包裹在生物惰性材料（琼脂糖）中的干细胞能够显著改善梗死小鼠心脏的功能。为了使用更具生物响应性的微胶囊，我们试图开发一种基于I型胶原的可酶降解微胶囊，用于在小鼠心肌梗死模型中介导骨髓间充质基质细胞的心肌内递送。
