Wu Shaohua, Ni Shilei, Jiang Xiping, Kuss Mitchell A, Wang Han-Jun, Duan Bin
College of Textiles & Clothing; Collaborative Innovation Center of Marine Biomass Fibers, Qingdao University, Qingdao 266071, China.
Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China.
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5284-5294. doi: 10.1021/acsbiomaterials.9b00748. Epub 2019 Sep 30.
Nerve guidance conduit (NGC)-infilling substrates have been reported to facilitate the regeneration of injured peripheral nerves (PNs), especially for large nerve gaps. In this study, longitudinally oriented electrospun core-sheath nanoyarns (csNYs), consisting of a polylactic acid microfiber core and an electrospun nanofiber sheath, were fabricated for potential PN tissue engineering applications. Our novel csNY displayed a well-aligned nanofibrous surface topography, resembling the ultrastructure of axons and fascicles of a native PN system, and it also provided a mechanically stable structure. The biological results showed that the csNY significantly enhanced the attachment, growth, and proliferation of human adipose derived mesenchymal stem cells (hADMSC) and also promoted the migration, proliferation, and phenotype maintenance of rabbit Schwann cells (rSCs). Our csNY notably increased the differentiation capability of hADMSC into SC-like cells (hADMSC-SC), in comparison with a 2D tissue culture polystyrene plate. More importantly, when combined with the appropriate induction medium, our csNY promoted hADMSC-SC to express high levels of myelination-associated markers. Overall, this study demonstrates that our csNYs have great potential to serve as not only ideal in vitro culture models for understanding SC-axon interaction and SC myelination but also as promising NGC-infilling substrates for PN regeneration applications.
据报道，神经导向导管（NGC）填充基质有助于损伤周围神经（PN）的再生，尤其是对于大的神经间隙。在本研究中，制备了由聚乳酸微纤维芯和电纺纳米纤维鞘组成的纵向取向的电纺核壳纳米纱（csNY），用于潜在的PN组织工程应用。我们的新型csNY呈现出排列良好的纳米纤维表面形貌，类似于天然PN系统的轴突和束状结构的超微结构，并且还提供了机械稳定的结构。生物学结果表明，csNY显著增强了人脂肪来源间充质干细胞（hADMSC）的附着、生长和增殖，还促进了兔雪旺细胞（rSCs）的迁移、增殖和表型维持。与二维组织培养聚苯乙烯板相比，我们的csNY显著提高了hADMSC向雪旺细胞样细胞（hADMSC-SC）的分化能力。更重要的是，当与适当的诱导培养基结合时，我们的csNY促进hADMSC-SC表达高水平的髓鞘相关标志物。总体而言，本研究表明，我们的csNY不仅有潜力作为理解雪旺细胞-轴突相互作用和雪旺细胞髓鞘形成的理想体外培养模型，而且有潜力作为PN再生应用中很有前景的NGC填充基质。
