Feng Fan, Wu Yulu, Xin Haitao, Chen Xiaoqiang, Guo Yazhou, Qin Dongyang, An Baili, Diao Xiaoou, Luo Huiwen
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Department of Plastic and Burn Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5107-5115. doi: 10.1021/acsbiomaterials.9b00579. Epub 2019 Sep 27.
This study investigated the surface characteristics and biocompatibility of ultrafine-grain pure titanium (UFG Ti) after sandblasting and acid etching (SLA) treatment to determine an effective method for modification of UFG Ti dental implants. The UFG Ti was processed by equal-channel angular pressing (ECAP). The micromorphology, roughness, and wettability of its surface were studied after SLA modification in different conditions. Rat bone marrow mesenchymal stem cells were subsequently seeded onto the specimens to evaluate the biocompatibility of cell adhesion, proliferation, and differentiation compared with commercially pure titanium (CP Ti). The results showed that surface characteristics of UFG Ti were affected by the pressure of sandblasting and acid etching time in addition to material properties. The favorable hierarchical porous structure that would benefit cell adhesion was formed on the UFG Ti surface when the pressure of sandblasting was 0.6 MPa and the acid etching time was 5 min; at this time, UFG Ti promoted proliferation and differentiation to a greater extent than CP Ti because of its excellent wettability. From this study, it could be seen that UFG Ti can be used as a dental implant material after proper surface modification.
本研究调查了喷砂和酸蚀（SLA）处理后超细晶粒纯钛（UFG Ti）的表面特性和生物相容性，以确定一种有效的UFG Ti牙科植入物改性方法。UFG Ti通过等通道转角挤压（ECAP）工艺制备。研究了不同条件下SLA改性后其表面的微观形态、粗糙度和润湿性。随后将大鼠骨髓间充质干细胞接种到标本上，以评估与商业纯钛（CP Ti）相比细胞粘附、增殖和分化的生物相容性。结果表明，除材料特性外，UFG Ti的表面特性还受喷砂压力和酸蚀时间的影响。当喷砂压力为0.6 MPa且酸蚀时间为5分钟时，UFG Ti表面形成了有利于细胞粘附的良好分级多孔结构；此时，由于其优异的润湿性，UFG Ti比CP Ti更能促进细胞增殖和分化。从本研究可以看出，经过适当的表面改性后，UFG Ti可作为牙科植入材料。
