Gallegos Silvia I, Parsaei Shaida, Siddiqui Danyal A, Biguetti Claudia C, Palmer Kelli L, Rodrigues Danieli C
Department of Biological Sciences, Bauru School of Dentistry, Bauru, São Paulo 17012, Brazil.
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5116-5127. doi: 10.1021/acsbiomaterials.9b00956. Epub 2019 Oct 1.
Cement-retained restorations on dental implants are a well-established method to replace missing teeth. However, undetected residual cement left during crown cementation procedures encourages microorganism growth, and it has been identified as a risk factor for peri-implant disease. Currently, there is no official guidance for dental cement selection, and the increasing variety of available compositions intensifies the complexity of the clinicians' decision process. The present study aimed to evaluate the in vitro host and bacterial cellular response to four different commercial dental cements as well as their effects on cement surface morphology. Disk specimens ( = 3) of bioceramic, zinc phosphate, resin-modified glass ionomer, and resin cements were exposed to host (murine pre-osteoblasts, human gingival fibroblasts, and undifferentiated human macrophages) and oral bacterial (, , , and ) cells. Results indicated that oral bacteria degraded the cement surface, but bacterial viability was not significantly affected by the presence of dental cement. Conversely, the biocompatibility and morphology of host cells were severely impacted by the cement composition. Only the bioceramic cement achieved >70% viability for all cell lines investigated. Within the limitations of this study, the results indicated the importance of considering the biological interactions of a dental cement composition during selection as it played a significant role in the host cellular response and the degree of surface degradation due to bacterial attack.
牙种植体上的水泥固位修复体是一种成熟的缺失牙替代方法。然而，在冠修复粘固过程中未检测到的残留水泥会促进微生物生长，并且已被确定为种植体周围疾病的一个风险因素。目前，对于牙科粘固剂的选择没有官方指导，可用成分种类的增加加剧了临床医生决策过程的复杂性。本研究旨在评估四种不同商业牙科粘固剂对体外宿主和细菌细胞的反应及其对粘固剂表面形态的影响。将生物陶瓷、磷酸锌、树脂改性玻璃离子和树脂粘固剂的圆盘标本（ = 3）暴露于宿主（小鼠前成骨细胞、人牙龈成纤维细胞和未分化的人巨噬细胞）和口腔细菌（、、、和）细胞。结果表明，口腔细菌会使粘固剂表面降解，但牙科粘固剂的存在对细菌活力没有显著影响。相反，宿主细胞的生物相容性和形态受到粘固剂成分的严重影响。在所研究的所有细胞系中，只有生物陶瓷粘固剂的活力>70%。在本研究的局限性范围内，结果表明在选择牙科粘固剂成分时考虑其生物相互作用的重要性，因为它在宿主细胞反应以及细菌攻击导致的表面降解程度中起着重要作用。
