Termteerapornpimol Kittipat, Tongchairati Karn, Intarak Narin, Prommanee Sasiprapa, Chantarangsu Soranun, Nantanapiboon Dusit, Tagami Junji, Porntaveetus Thantrira
Center of Excellence in Genomics and Precision Dentistry, Clinical Research Center, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand; Oral Biology Program, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Center of Excellence in Genomics and Precision Dentistry, Clinical Research Center, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Int Dent J. 2025 Apr;75(2):1047-1057. doi: 10.1016/j.identj.2024.11.004. Epub 2024 Dec 12.
Self-adhesive resin cements (SARCs) are widely used for fixed prostheses. These cements and their eluted products may affect periodontal tissues. This study aimed to investigate the response of human gingival fibroblasts (HGFs) to eluates from SARCs in vitro, simulating clinical conditions after prosthesis fixation, to gain insights into their potential effects on gingival health.
Two SARCs, RelyX U200 (RX) and Maxcem Elite Chroma (MC), were polymerised according to the manufacturer's protocols using various curing methods (light-cured, light-cured through 1- or 2 mm zirconia, and self-cured). HGFs were exposed to cement eluates at different concentrations. Cell viability, vitality, wound healing, and gene expression were assessed at different time points.
Self-cured MC and MC cured through 2-mm zirconia (both undiluted and 1:5 dilution) significantly decreased HGFs' viability. Lower cell viability and vitality were detected in MC compared with RX. Wound healing was delayed in cells treated with MC cured through zirconia compared to those cured with direct light, whereas zirconia had no effect on cells treated with RX. The expression of NRF2, a key regulator of cellular defence against oxidative and toxic insults, showed an increasing trend in cells treated with MC compared to RX. This finding suggests that MC may induce more oxidative stress than RX, leading to a more pronounced inflammatory response in HGFs and aligning with the observed delay in wound healing.
The use of MC, especially when cured through zirconia, may negatively impact gingival tissue health, highlighting the importance of carefully selecting cement types and curing methods in clinical practice.
This study highlights the potential risks of using MC, especially when cured through zirconia, which may impair gingival tissue health and delay wound healing. Proper choice of cement and curing methods is essential for optimal patient outcomes.
自粘树脂水门汀（SARCs）广泛应用于固定修复体。这些水门汀及其洗脱产物可能会影响牙周组织。本研究旨在体外模拟修复体固定后的临床情况，研究人牙龈成纤维细胞（HGFs）对SARCs洗脱液的反应，以深入了解其对牙龈健康的潜在影响。
两种自粘树脂水门汀，RelyX U200（RX）和Maxcem Elite Chroma（MC），按照制造商的方案采用不同的固化方法（光固化、通过1或2毫米氧化锆光固化以及自固化）进行聚合。将HGFs暴露于不同浓度的水门汀洗脱液中。在不同时间点评估细胞活力、活性、伤口愈合和基因表达。
自固化的MC以及通过2毫米氧化锆固化的MC（未稀释和1:5稀释）均显著降低了HGFs的活力。与RX相比，MC检测到较低的细胞活力和活性。与直接光固化处理的细胞相比，用通过氧化锆固化的MC处理的细胞伤口愈合延迟，而氧化锆对用RX处理的细胞没有影响。NRF2是细胞抵御氧化和毒性损伤的关键调节因子，其表达在MC处理的细胞中与RX相比呈上升趋势。这一发现表明，MC可能比RX诱导更多的氧化应激，导致HGFs中更明显的炎症反应，并与观察到的伤口愈合延迟一致。
使用MC，尤其是通过氧化锆固化时，可能会对牙龈组织健康产生负面影响，突出了在临床实践中仔细选择水门汀类型和固化方法的重要性。
本研究强调了使用MC的潜在风险，尤其是通过氧化锆固化时，这可能会损害牙龈组织健康并延迟伤口愈合。正确选择水门汀和固化方法对于获得最佳患者预后至关重要。
