Wang Lingren, Gong Tao, Brown Zachary, Randle Christopher, Guan Yingying, Ye Wei, Ming Weihua
Department of Mechanical and Materials Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
Department of Chemistry and Biochemistry, Georgia Southern University, P.O. Box 8064, Statesboro, Georgia 30460, United States.
ACS Biomater Sci Eng. 2020 Apr 13;6(4):1998-2006. doi: 10.1021/acsbiomaterials.9b01865. Epub 2020 Mar 24.
In the present study, heparin-mimetic magnetic nanoparticles (HMNPs), which might be used as recycling anticoagulants, were synthesized by coating heparin-mimetic sodium alginate (HLSA) on the surface of iron oxide magnetic nanoparticles (MNPs), using 3,4,5-trihydroxyphenylalanine (TOPA) as a biological adhesive. HLSA was successfully immobilized on the MNP surface, as revealed by Fourier transform infrared spectroscopy and thermal gravimetric analysis, and the core (MNP)-shell (TOPA, HLSA) structure was confirmed by transmission electron microscopy observations. In addition, in vitro studies of protein adsorption, blood clotting time, and contact activation confirmed that the blood compatibility of the HMNP was significantly enhanced compared with the bare MNP. The improved hemocompatibility was attributed to the introduction of the multiple heparin-mimetic groups (-SONa, -COONa, and -OH). In addition, the HMNP showed outstanding recycle stability and, thus, can be reused if needed. The synthesized HMNP appeared to be a suitable biomaterial to safely replace heparin as an anticoagulant in patients undergoing long-term hemodialysis.
在本研究中，通过使用3,4,5-三羟基苯丙氨酸（TOPA）作为生物粘合剂，将肝素模拟海藻酸钠（HLSA）包覆在氧化铁磁性纳米颗粒（MNPs）表面，合成了可作为循环抗凝剂的肝素模拟磁性纳米颗粒（HMNPs）。傅里叶变换红外光谱和热重分析表明HLSA成功固定在MNP表面，透射电子显微镜观察证实了核（MNP）-壳（TOPA，HLSA）结构。此外，蛋白质吸附、凝血时间和接触激活的体外研究证实，与裸MNP相比，HMNP的血液相容性显著提高。血液相容性的改善归因于多个肝素模拟基团（-SONa、-COONa和-OH）的引入。此外，HMNP表现出出色的循环稳定性，因此可根据需要重复使用。合成的HMNP似乎是一种合适的生物材料，能够安全替代肝素，用于长期血液透析患者的抗凝治疗。
