Xiang MingLi, Zhu Mengyuan, Yang Zun, He Ping, Wei Jingjing, Gao Xiang, Song Jinlin
College of Stomatology, Chongqing Medical University, Chongqing 401147, China.
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China.
ACS Biomater Sci Eng. 2020 Mar 9;6(3):1704-1714. doi: 10.1021/acsbiomaterials.9b01893. Epub 2020 Feb 24.
The development of biomimetic bone graft materials for periodontal tissue engineering is a field of topical interest. In this study, we designed a dual-functionalized apatite nanocomposite, which could integrate multiple molecular cues for manipulating the fate of periodontal ligament stem cells (PDLSCs). Briefly, inspired by mussels, a biomimetic nanohydroxyapatite was fabricated using a polydopamine structure as a template (named as tHA) and then surface-modified with bone-forming peptide-1 (BFP-1) and vascular endothelial growth factor-mimicking peptide (QK) via a single step of catechol chemistry. Our study showed that the biofunctions of tethered peptides were not compromised on the surface of apatite nanoparticles. Because of the synergistic effect of BFP-1 and QK peptides, the dual-functionalized apatite nanocomposite showed improved cytocompatibility compared to controls. Moreover, it can boost the proliferation and osteogenic differentiation of PDLSCs, indicating excellent bioactivity of tHA-BFP/QK nanoparticles on cell fate decision. More importantly, animal experiments showed that dual-functionalized apatite nanocomposites could dramatically promote the regeneration of periodontal bone. It is concluded that our work provides an instructive insight into the design of biomimetic apatite nanocomposites, which holds a great potential for applications in periodontal bone repair.
用于牙周组织工程的仿生骨移植材料的开发是一个备受关注的领域。在本研究中，我们设计了一种双功能化的磷灰石纳米复合材料，它可以整合多种分子信号来调控牙周膜干细胞（PDLSCs）的命运。简而言之，受贻贝启发，以聚多巴胺结构为模板制备了一种仿生纳米羟基磷灰石（命名为tHA），然后通过一步儿茶酚化学法用骨形成肽-1（BFP-1）和血管内皮生长因子模拟肽（QK）对其进行表面修饰。我们的研究表明，连接在磷灰石纳米颗粒表面的肽的生物功能没有受到损害。由于BFP-1和QK肽的协同作用，与对照相比，双功能化的磷灰石纳米复合材料表现出更好的细胞相容性。此外，它可以促进PDLSCs的增殖和成骨分化，表明tHA-BFP/QK纳米颗粒在细胞命运决定方面具有优异的生物活性。更重要的是，动物实验表明双功能化的磷灰石纳米复合材料可以显著促进牙周骨的再生。结论是，我们的工作为仿生磷灰石纳米复合材料的设计提供了有指导意义的见解，其在牙周骨修复中的应用具有巨大潜力。
