Thomas J B, Crews S T, Goodman C S
Department of Biological Sciences, Stanford University, California 94305.
Cell. 1988 Jan 15;52(1):133-41. doi: 10.1016/0092-8674(88)90537-5.
The embryonic neuroepithelium of Drosophila gives rise to the central nervous system. We have studied the mutant phenotype and expression of a gene, single-minded (sim), which is involved in generating a specific region of this neuroepithelium. In sim mutant embryos, a subset of neuronal and nonneuronal precursor cells lying along the midline fail to emerge with the rest of the neuroepithelium. We have identified the sim transcription unit and have shown by in situ hybridization to embryos that the sim gene is expressed specifically in the midline neuroepithelium. Both the mutant phenotype and the temporal and spatial expression of transcripts suggest that the sim gene plays a key role in the emergence of this subset of cells along the midline of the developing central nervous system.
果蝇的胚胎神经上皮会发育成中枢神经系统。我们研究了一个名为“单 minded”（sim）的基因的突变表型和表达情况，该基因参与了这一神经上皮特定区域的形成。在 sim 突变胚胎中，位于中线的一部分神经元和非神经元前体细胞无法与神经上皮的其他部分一起出现。我们已经鉴定出 sim 转录单位，并通过对胚胎进行原位杂交表明，sim 基因在中线神经上皮中特异性表达。突变表型以及转录本的时空表达都表明，sim 基因在发育中的中枢神经系统中线处这一细胞亚群的出现过程中起着关键作用。
