Li Yang, Shi Huacai, Chen Rui, Zhou Shanyao, Lei Si, She Yanling
Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China.
Exp Ther Med. 2021 Feb;21(2):146. doi: 10.3892/etm.2020.9577. Epub 2020 Dec 16.
Skeletal muscle atrophy is a well-known adverse effect of long-term glucocorticoid (GC) therapy. MicroRNAs (miRNAs or miRs) and long non-coding RNAs (lncRNAs) are important regulators in a number of physiological and pathological processes. However, the role of miRNAs and lncRNAs in the regulation of GC-treated muscle atrophy remains poorly understood. In the current study, muscular atrophy was induced and the results indicated that C2C12 myotubes were thinner than normal, while the expression of muscle ring finger protein 1 and Atrogin-1 was increased. The expression of nine miRNAs and seven lncRNAs associated with proliferation and differentiation were analyzed in a dexamethasone (DEX)-induced muscle atrophy cell model. In addition, the mRNA expression of the downstream targets of lncRNAs that were differentially expressed between DEX-treated and control cells were determined. The results indicated that the expression of miR-133a, miR-133b, miR-206 and five lncRNAs (increased Atrolnc-1, Dum, MAR1, linc-MD1 and decreased Myolinc) were significantly different between the DEX and the control group. Furthermore, the relative mRNA expression of Wnt5a and MyoD was significantly different between the two groups. The results of the current study indicated that some important miRNAs and lncRNAs are associated with DEX-induced muscle atrophy and have the potential to be further developed as a diagnostic tool for this condition.
骨骼肌萎缩是长期糖皮质激素（GC）治疗众所周知的不良反应。微小RNA（miRNA或miR）和长链非编码RNA（lncRNA）是许多生理和病理过程中的重要调节因子。然而，miRNA和lncRNA在糖皮质激素治疗引起的肌肉萎缩调节中的作用仍知之甚少。在本研究中，诱导了肌肉萎缩，结果表明C2C12肌管比正常肌管更细，而肌肉环指蛋白1和Atrogin-1的表达增加。在一个地塞米松（DEX）诱导的肌肉萎缩细胞模型中，分析了与增殖和分化相关的9种miRNA和7种lncRNA的表达。此外，还测定了DEX处理细胞和对照细胞之间差异表达的lncRNA下游靶标的mRNA表达。结果表明，DEX组和对照组之间miR-133a、miR-133b、miR-206和5种lncRNA（Atrolnc-1、Dum、MAR1、linc-MD1增加，Myolinc减少）的表达有显著差异。此外，两组之间Wnt5a和MyoD的相对mRNA表达也有显著差异。本研究结果表明，一些重要的miRNA和lncRNA与DEX诱导的肌肉萎缩有关，有潜力进一步开发成为这种疾病的诊断工具。
