Wu Jing, Li Qiang, Deng Jiaxin, Zhao Jin-Jun, Yu Qing-Hong
Rheumatology and Clinical Immunology Department, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
Rheumatology and Clinical Immunology Department, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
Exp Ther Med. 2021 Feb;21(2):161. doi: 10.3892/etm.2020.9592. Epub 2020 Dec 18.
IL-33 is a member of the IL-1 family of cytokines whose role remains controversial in rheumatoid arthritis (RA). The present study was performed to evaluate the correlation of IL-33 with other cytokines and chemokines in serum and the synovia, and to explore the nature of the association. The concentration of IL-33 in samples from 96 patients with RA was analyzed. The response of fibroblast-like synoviocytes (FLSs) to treatment with different concentrations of IL-33 was assessed . IL-33 was indicated to exhibit an association with multiple cytokines and chemokines in synovial fluid with an inverted-U-shaped trend, including IL-6, IL-1β, IL-8, MIG and IP-10, but not in the serum. Furthermore, experiments confirmed that IL-33 also exerted a U-type dose-dependent regulatory effect on FLS function. In addition, the data-points do not exactly follow the U-shaped curve fit in most cases, therefore, the applicability of this mathematical model in clinical practice is limited.
白细胞介素-33（IL-33）是细胞因子白细胞介素-1家族的成员，其在类风湿性关节炎（RA）中的作用仍存在争议。本研究旨在评估血清和滑膜中IL-33与其他细胞因子和趋化因子的相关性，并探讨这种关联的性质。分析了96例RA患者样本中IL-33的浓度。评估了成纤维样滑膜细胞（FLS）对不同浓度IL-33处理的反应。结果表明，IL-33与滑液中的多种细胞因子和趋化因子呈倒U形趋势相关，包括白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）、γ干扰素诱导的单核因子（MIG）和干扰素诱导蛋白10（IP-10），但在血清中无此关联。此外，实验证实IL-33对FLS功能也具有U型剂量依赖性调节作用。另外，在大多数情况下数据点并不完全符合U形曲线拟合，因此该数学模型在临床实践中的适用性有限。
