Soshnikova N V, Sheynov A A, Tatarskiy Eu V, Georgieva S G
Institute of Gene Biology Russian Academy of Sciences, Moscow, 119334 Russia.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Acta Naturae. 2020 Oct-Dec;12(4):57-65. doi: 10.32607/actanaturae.11092.
The DPF (double PHD finger) domain consists of two PHD fingers organized in tandem. The two PHD-finger domains within a DPF form a single structure that interacts with the modification of the N-terminal histone fragment in a way different from that for single PHD fingers. Several histone modifications interacting with the DPF domain have already been identified. They include acetylation of H3K14 and H3K9, as well as crotonylation of H3K14. These modifications are found predominantly in transcriptionally active chromatin. Proteins containing DPF belong to two classes of protein complexes, which are the transcriptional coactivators involved in the regulation of the chromatin structure. These are the histone acetyltransferase complex belonging to the MYST family and the SWI/SNF chromatin-remodeling complex. The DPF domain is responsible for the specificity of the interactions between these complexes and chromatin. Proteins containing DPF play a crucial role in the activation of the transcription of a number of genes expressed during the development of an organism. These genes are important in the differentiation and malignant transformation of mammalian cells.
双PHD指（DPF）结构域由两个串联排列的PHD指组成。DPF中的两个PHD指结构域形成一个单一结构，该结构与N端组蛋白片段的修饰相互作用，其方式不同于单个PHD指。已经鉴定出几种与DPF结构域相互作用的组蛋白修饰。它们包括H3K14和H3K9的乙酰化，以及H3K14的巴豆酰化。这些修饰主要存在于转录活跃的染色质中。含有DPF的蛋白质属于两类蛋白质复合物，它们是参与染色质结构调节的转录共激活因子。这些是属于MYST家族的组蛋白乙酰转移酶复合物和SWI/SNF染色质重塑复合物。DPF结构域负责这些复合物与染色质之间相互作用的特异性。含有DPF的蛋白质在生物体发育过程中表达的许多基因的转录激活中起关键作用。这些基因在哺乳动物细胞的分化和恶性转化中很重要。
