Wang Yong, Li Wei, Guo Xu, Zhang Da, Sun Jianfei, Fu Ziteng, Liu Guangqing, Li Yongdong, Jiang Shudong
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 People's Republic of China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241 People's Republic of China.
3 Biotech. 2021 Feb;11(2):61. doi: 10.1007/s13205-020-02577-8. Epub 2021 Jan 11.
Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detection limit of the qPCR was 3.87 × 10 copies/μL. Of note, the detection limit of the conventional polymerase chain reaction (cPCR) was 3.87 × 10 copies/μL. The highest intra-assay and inter-assay coefficients of variation (CV%) were 0.98% and 1.42%, respectively. The positive detection rate of 128 clinical samples using the qPCR and the cPCR was 7.0% (9/128) and 4.7% (6/128), respectively. Taken together, these results indicated that the established qPCR assay has good sensitivity, high specificity, and good reproducibility. Therefore, it could provide support for the rapid and efficient clinical detection of FBoV-1.
猫博卡病毒1型（FBoV-1）可能与猫的腹泻有关。在本研究中，建立了一种基于SYBR Green I的定量聚合酶链反应（qPCR）检测方法来检测FBoV-1。熔解曲线显示在83.0℃有一个单一的熔解峰。敏感性结果表明，qPCR的检测限为3.87×10拷贝/μL。值得注意的是，传统聚合酶链反应（cPCR）的检测限为3.87×10拷贝/μL。测定内和测定间的最高变异系数（CV%）分别为0.98%和1.42%。使用qPCR和cPCR对128份临床样本的阳性检出率分别为7.0%（9/128）和4.7%（6/128）。综上所述，这些结果表明所建立的qPCR检测方法具有良好的敏感性、高特异性和良好的重复性。因此，它可为FBoV-1的快速高效临床检测提供支持。
