Liu Xunbi, Li Shuyan, Liu Xuan, Wang Run, Xie Xiangyu, Wu Haiqiang, Wang Yong
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 People's Republic of China.
3 Biotech. 2022 Apr;12(4):91. doi: 10.1007/s13205-022-03150-1. Epub 2022 Mar 14.
Feline parvovirus causes infectious diseases, and Chaphamaparvovirus is a novel type of feline parvovirus. The present study aims to establish a method that can be used in clinical rapid detection of feline Chaphamaparvovirus (FeChPV), for facilitate the timely and effective diagnosis and treatment of sick animals and shorten the diagnosis time of clinical diseases. The experimental samples in this study are from 20 cats undergoing physical examination in Hefei Xin'an Animal Hospital. An SYBR Green I-based qPCR assay was performed to detect FeChPV. A pair of specific primers was designed based on the gene to perform the assay. The detection assay showed high sensitivity with a detection limit of 1.07 × 10 copies/μL and high specificity for detection of only the target virus. The coefficients of value variation were calculated to assess the reproducibility of the qPCR assay, and the inter- and intra-assay ranged from 0.21 to 0.67% and 0.10 to 0.56%, respectively. The result of clinical sample detection showed that the infection rate of FeChPV in 124 samples detected using qPCR assay was higher than that with conventional PCR. The established qPCR assay could be a low-cost, convenient, and reliable method to detect FeChPV in clinical practice.
猫细小病毒可引发传染性疾病，而查帕马细小病毒是一种新型猫细小病毒。本研究旨在建立一种可用于临床快速检测猫查帕马细小病毒（FeChPV）的方法，以促进患病动物的及时有效诊断与治疗，并缩短临床疾病的诊断时间。本研究中的实验样本来自合肥新安动物医院接受体检的20只猫。采用基于SYBR Green I的qPCR检测法检测FeChPV。基于该基因设计了一对特异性引物来进行检测。该检测法显示出高灵敏度，检测限为1.07×10拷贝/微升，且对仅检测目标病毒具有高特异性。计算 值变异系数以评估qPCR检测法的重复性，批间和批内变异系数分别为0.21%至0.67%和0.10%至0.56%。临床样本检测结果表明，使用qPCR检测法检测的124份样本中FeChPV的感染率高于传统PCR检测法。所建立的qPCR检测法可能是临床实践中检测FeChPV的一种低成本、便捷且可靠的方法。
