Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Intervirology. 2018;61(5):230-236. doi: 10.1159/000495181. Epub 2019 Jan 17.
To establish an efficient, convenient and quantitative method for the clinical detection of the duck-origin goose parvovirus.
In the present study, a real-time polymerase chain reaction (PCR) method was established for detecting the duck-origin goose parvovirus using the fluorescent chimeric dye SYBR Green II. Specific primers were designed to target a highly conserved region of the VP3 gene of the duck-origin goose parvovirus.
This method was able to detect a minimum of 19.6 copies/μL of viral genomic DNA. Results showed that this method was faster and had a higher sensitivity than the traditional PCR in the clinical specimen test. In this paper, we developed a rapid, sensitive detection and quantitative analysis technology for the duck-origin goose parvovirus by real-time PCR assay.
This test provides improved technical support for studies regarding the clinical diagnosis and epidemiological investigations of the duck-origin goose parvovirus.
建立一种高效、便捷、定量的鸭源鹅细小病毒临床检测方法。
本研究采用荧光嵌合染料 SYBR Green II 建立了实时聚合酶链反应（PCR）方法来检测鸭源鹅细小病毒。设计了针对鸭源鹅细小病毒 VP3 基因高度保守区的特异性引物。
该方法最低可检测到 19.6 拷贝/μL 的病毒基因组 DNA。结果表明，与传统 PCR 相比，该方法在临床标本检测中更快、更灵敏。本文通过实时 PCR 法建立了一种快速、灵敏的鸭源鹅细小病毒检测和定量分析技术。
该试验为鸭源鹅细小病毒的临床诊断和流行病学调查研究提供了改进的技术支持。
