Fazel Torshizi Faeze, Chamani Mohammad, Khodaei Hamid Reza, Sadeghi Ali Asghar, Hejazi Seyed Hossein, Heravi Reza Majidzadeh
Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Animal Sciences, Islamic Azad University, Golpayegan Branch, Tehran, Iran.
Adv Biomed Res. 2020 Oct 30;9:57. doi: 10.4103/abr.abr_144_20. eCollection 2020.
Polycystic ovarian syndrome (PCOS) is an endocrine disorder in fertile women, which seems to be adversely affected by associated thyroid dysfunction. Zinc methionine (ZM) has positive effects on PCOS, but its concerted effects on PCOS and thyroid function have not been investigated. We evaluated the effects of ZM on reproductive and thyroid hormones and the number of follicles in rats with PCOS.
This study was conducted on 45 female rats, using sesame oil as control; PCOS animals administered with 0, 25, 75, and 175 mg/kg BW of ZM. Serum concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and thyroid function were investigated. Premature follicles (PMF), primary follicles (PF), preantral follicles (PAF), antral follicles (AF), corpus luteum (CL), and cystic follicles (CF) were assessed.
PCOS decreased the concentrations of FSH and free T4, but increased the levels of LH, TSH, and LH/FSH ratio (ALL < 0.05). ZM at a dose of 75 and 175 mg increased the level of FSH, free T4, and decreased LH, TSH, and LH/FSH ratio (ALL < 0.05). Induction of PCOS decreased PMF, PF, PAF, AF, and CL, but increased CF ( < 0.05). PCOS treated groups (75 and 175 mg/kg) increased these follicle numbers and decreased CF compared to ZM 25 mg/kg and PCOS groups (Both < 0.05).
Although the induction of PCOS had a negative effect on reproductive and thyroid hormones and follicle numbers, ZM treatment (75 and 175 mg/kg) overcame the negative effects. A high dosage of ZM can alleviate the hormonal and cysts disturbances occurring in PCOS.
多囊卵巢综合征（PCOS）是育龄女性的一种内分泌紊乱疾病，似乎会受到相关甲状腺功能障碍的不利影响。蛋氨酸锌（ZM）对PCOS有积极作用，但其对PCOS和甲状腺功能的协同作用尚未得到研究。我们评估了ZM对PCOS大鼠生殖和甲状腺激素以及卵泡数量的影响。
本研究以45只雌性大鼠为对象，以芝麻油作为对照；PCOS动物分别给予0、25、75和175mg/kg体重的ZM。检测血清促卵泡生成素（FSH）、黄体生成素（LH）浓度以及甲状腺功能。评估早熟卵泡（PMF）、初级卵泡（PF）、窦前卵泡（PAF）、窦卵泡（AF）、黄体（CL）和囊性卵泡（CF）。
PCOS降低了FSH和游离T4的浓度，但升高了LH、TSH水平以及LH/FSH比值（均P<0.05）。75mg和175mg剂量的ZM可提高FSH、游离T4水平，并降低LH、TSH水平以及LH/FSH比值（均P<0.05）。诱导PCOS可减少PMF）、PF、PAF、AF和CL数量，但增加CF数量（P<0.05）。与25mg/kg ZM组和PCOS组相比，PCOS治疗组（75mg/kg和175mg/kg）增加了这些卵泡数量并减少了CF数量（均P<0.05）。
尽管诱导PCOS对生殖和甲状腺激素以及卵泡数量有负面影响，但ZM治疗（75mg/kg和175mg/kg）克服了这些负面影响。高剂量的ZM可缓解PCOS中出现的激素和囊肿紊乱。
