Baldion Paula Alejandra, Velandia-Romero Myriam L, Castellanos Jaime E
Grupo de Investigaciones Básicas y Aplicadas en Odontología, Departamento de Salud Oral, Facultad de Odontología, Universidad Nacional de Colombia, Bogotá, Colombia.
Grupo de Virología, Universidad El Bosque, Bogotá, Colombia.
Data Brief. 2020 Dec 23;34:106684. doi: 10.1016/j.dib.2020.106684. eCollection 2021 Feb.
Data in this article are associated with our research article "Dental Resin Monomers Induce Early and Potent Oxidative Damage on Human Odontoblast-like Cells." Dental adhesives are polymeric compounds consisting of several chemical substances, including resin monomers, such as 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA), together with other comonomers, making up the organic matrix of the adhesive and whose composition is based on the methyl methacrylate chemistry. The release of residual monomers, susceptible to biodegradation, acts as a source of bioactive compounds, which can interact with tissues and induce a cytotoxic cellular response. The most used techniques to evaluate cytotoxicity, proliferation, or metabolic activity of cells exposed to different substances, are MTT and resazurin. Each chemistry evaluates cell viability differently, so the data obtained could vary depending on the technique sensitivity to detect changes in cell metabolism. The objective of this article was to present viability data as a function of the metabolic activity in human odontoblast-like cells (hOLCs), exposed to 3, 6, 9, and 12 mM HEMA, or 0.75, 1.5, 3, and 6 mM TEGDMA evaluated by the MTT, and resazurin techniques in the first 24 hours of exposure, at different time points. The absorbance data for the MTT test and the fluorescence intensity for the resazurin test were obtained by spectrometry. SIMSTAT software 2.6.5 for Windows was used to confirm the normal data distribution (Levene's test). Subsequently, an analysis of variance (one-way ANOVA) was performed to compare the control with each HEMA and TEGDMA concentration. Where a < 0.05 indicated a high F value, a Fisher's least significant differences post-hoc analysis was performed, using an alpha value < 0.05. Data from the different time points were compared with a Student's t-test for each concentration. These data may be useful to compare the cytotoxic response of hOLCs with other cell types or the cell response to other resin monomers.
本文中的数据与我们的研究论文《牙科树脂单体对人成牙本质细胞样细胞的早期和强效氧化损伤》相关。牙科粘合剂是由几种化学物质组成的聚合物化合物，包括树脂单体，如甲基丙烯酸2-羟乙酯（HEMA）和二缩三乙二醇二甲基丙烯酸酯（TEGDMA），以及其他共聚单体，构成粘合剂的有机基质，其组成基于甲基丙烯酸甲酯化学。易生物降解的残留单体的释放作为生物活性化合物的来源，其可与组织相互作用并诱导细胞毒性细胞反应。评估暴露于不同物质的细胞的细胞毒性、增殖或代谢活性的最常用技术是MTT和刃天青。每种化学方法对细胞活力的评估方式不同，因此获得的数据可能会因检测细胞代谢变化的技术敏感性而异。本文的目的是呈现人成牙本质细胞样细胞（hOLCs）中作为代谢活性函数的活力数据，该细胞暴露于3、6、9和12 mM的HEMA，或0.75、1.5、3和6 mM的TEGDMA，通过MTT和刃天青技术在暴露的前24小时内的不同时间点进行评估。MTT试验的吸光度数据和刃天青试验的荧光强度通过光谱法获得。使用适用于Windows的SIMSTAT软件2.6.5来确认数据的正态分布（Levene检验）。随后，进行方差分析（单因素方差分析）以将对照组与每种HEMA和TEGDMA浓度进行比较。当p < 0.05表示F值较高时，使用α值< 0.05进行Fisher最小显著差异事后分析。将来自不同时间点的数据与每种浓度的学生t检验进行比较。这些数据可能有助于比较hOLCs与其他细胞类型的细胞毒性反应或细胞对其他树脂单体的反应。
