Wen Yangmin, Wan Duanjing
Quanzhou Medical College, Quanzhou, China.
Quanzhou Hospital of Traditional Chinese Medicine, Quanzhou, China.
Mitochondrial DNA B Resour. 2020 Jul 9;5(3):2713-2715. doi: 10.1080/23802359.2020.1787896.
To understand the process of chloroplast genome evolution, information on repeated sequences, intergenic regions, and pseudogenes in chloroplast DNA is extremely helpful. is a fast-growing fresh-water microalga cultivated on the industrial scale for applications ranging from food to biofuel production. Structure and expression of the chloroplast genome have been studied in a number of plants. Gene content and the sequence of many genes in chloroplast DNA are relatively conserved among land plants and the Euglenophyta Euglena gracilis. The complete chloroplast genome sequence of was characterized from Illumina pair-end sequencing. The chloroplast genome of was 165,412 bp in length and the genome contains no large inverted repeat and has one copy of rRNA gene cluster consisting of 16S, 23S, and 5S rRNA genes. And the genes with two exons containing rrn23 (rRNA), trnl-UAA (tRNA), psba (CDS) and chlL (CDS). The genome contains 114 complete genes, including 78 protein-coding genes (45 protein-coding gene species), 33 tRNA genes (26 tRNA species), and 3 rRNA genes (3 rRNA species). The neighbour-joining phylogenetic analysis showed that and NC001865 clustered together as sisters to other species.
为了解叶绿体基因组的进化过程，叶绿体DNA中重复序列、基因间区域和假基因的信息非常有帮助。是一种快速生长的淡水微藻，在工业规模上进行培养，应用范围从食品到生物燃料生产。已经在许多植物中研究了叶绿体基因组的结构和表达。叶绿体DNA中的基因含量和许多基因的序列在陆地植物和裸藻门纤细裸藻中相对保守。通过Illumina双末端测序对的完整叶绿体基因组序列进行了表征。的叶绿体基因组长度为165,412 bp，该基因组不包含大的反向重复序列，有一个由16S、23S和5S rRNA基因组成的rRNA基因簇拷贝。并且具有两个外显子的基因包含rrn23（rRNA）、trnl-UAA（tRNA）、psba（CDS）和chlL（CDS）。该基因组包含114个完整基因，包括78个蛋白质编码基因（45种蛋白质编码基因）、33个tRNA基因（26种tRNA）和3个rRNA基因（3种rRNA）。邻接法系统发育分析表明，和NC001865聚在一起，作为其他物种的姐妹。
